Comparing and Analyzing the Disease Resistances of Four Disease-resistant Selected Lines of Macrobrachium rosenbergii

doi:10.13560/J.cnki.biotech.bull.1985.2017-0096

Abstract

Abstract:

Expressions of 4 disease-resistant genes（heat shock protein70（HSP70），Se-GPx，CAT，and SOD）from 4 selected lines（A，B，C，and D）of Macrobrachium rosenbergii were analyzed with real-time PCR. Infected with Vibrio alginolyticus，the disease-resistant abilities of the 4 selected lines were compared based on the survival rates of 4 selected lines and the expression differences ofHSP70，Se-GPx，CAT，and SOD among the 4 selected lines. The results showed that relative expression of HSP70 mRNA of A and B was obviously higher than that of C and D in the hepatopancreas（P<0.05）. The expressions of Se-GPx，CAT，and SOD changed significantly within 96 h，and were different among the 4 selected lines at different time；however，the general trend was that expressions in A and B were higher than those in C and D. The rate of A，B，C，and D was 65.0%，65.8%，51.7%，and 53.3% after infecting with V.alginolyticus for a week，it is inferred that disease-resistant ability of A and B is higher than that of C and D.

Key words: Macrobrachium rosenbergii, HSP70, non-specific immune, Vibrio alginolyticus

LI Yu-feng, DAI Xi-lin,YUAN Xin-cheng,ZHOU Xun,HU Yan-jie,DING Fu-jiang. Comparing and Analyzing the Disease Resistances of Four Disease-resistant Selected Lines of Macrobrachium rosenbergii[J]. Biotechnology Bulletin, 2017, 33(7): 203-209.

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