Cloning,Expression and Characterization of the Esterase Gene estZ from Vibrio alginolyticus

doi:10.13560/j.cnki.biotech.bull.1985.2017-0028

Abstract

Abstract: This work aimed to investigate the esterase gene estZ of Vibrio alginolyticus 7S-1,which was expressed in Escherichia coli and characterized. The estZ gene was obtained from the genomic DNA of V. alginolyticus 7S-1 by gene cloning,then ligated to the vector pET28a,and expressed in E. coli BL21（DE3）. E. coli BL21-28a-estZ expressed the estZ gene with esterase activity. The specific activity of the purified protein EstZ reached 5.42 U/μg when the E. coli cells were induced by 0.4 mmol/L IPTG and grew at 18℃ for 22 hours. The optimal temperature and pH of the EstZ protein were 25℃ and 9.0,respectively. EstZ was activated by 10 mmol/L Na⁺ and Mg²⁺,but highly inhibited by Cu²⁺,Ni²⁺ and Co²⁺. In addition,EstZ is resistant to some organic solvents,surfactants and salt. This study would provide some insights of V. alginolyticus in biological deinking industry.

Key words: Vibrio alginolyticus, esterase, enzymatic property

ZHAO Shu-mei, WANG Lin-hui, TANG Jia-qi, ZHAO Jing-yi. Cloning,Expression and Characterization of the Esterase Gene estZ from Vibrio alginolyticus[J]. Biotechnology Bulletin, 2017, 33(6): 190-196.

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