Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (12): 133-140.doi: 10.13560/j.cnki.biotech.bull.1985.2014.12.022

• Research Report • Previous Articles     Next Articles

Influence of Piriformospora indica on Host Plant Selection by Aphid Lipaphis erysimi(Kaltenbach)

Liu Jinhua, Wang Ting, Gao Qikang   

  1. (Instituteof Insect Sciences, Zhejiang University, Hangzhou 310058)
  • Received:2014-05-14 Online:2014-12-08 Published:2014-12-12

Abstract: In order to confirm the influence of Piriformospora indica on host plant selection of L. erysimi. Aphid dual-choice assay was taken under greenhouse and artificial control environment and the amounts of aphids on the P. indica-colonized B. napus was significantly less than the P. indica-free B. napus. Volatiles emitted by plants collected by dynamic headspace systems, the quality and quantitative differences of volatiles emitted by B. napus were analyzed through GC-MS. The result indicated that P. indica inoculated B. napus and P. indica-free B. napus had the same volatiles, but the quantity of total volatiles, α-pinene, terpinolene, camphor, β-thujene, cedrene and β-caryophyllene emitted by B. napus with P. indica were significantly higher than P. indica-free B. napus in unit time. Through the four-sector olfactomete method, we confirmed that the α-pinene significantly attracted the L. erysimi, while the camphor significantly repelled the L. erysimi. And the mixture of camphor and α-pinene(2∶1)was also significantly repelled the L. erysimi. Terpinolene, β-thujene, cedrene and β-caryophyllene had no effect on the L. erysimi.

Key words: Piriformospora indica, Lipaphis erysimi, Host plant selection, Volatile