Antioxidant Activity,Antibacterial Activity and Volatile Components of Extracts from the Branches and Leaves of Torreya fargesii Franch.

doi:10.13560/j.cnki.biotech.bull.1985.2021-0130

Abstract

Abstract:

Our aims are to explore the antioxidant capacity,bacteriostatic activity,and volatile components of the extracts from the branches and leaves of T. grandis,and to provide scientific basis for the development and utilization of the edible or medicinal value of T. fargesii Franch. The exactions from the branches and leaves of T. fargesii Franch. were conducted by 90% ethanol,antioxidant capacity was determined using free radical scavenging rate,antibacterial activity was analyzed by Oxford Cup Method,and volatile essential components and their relative contents were detected by head sead spacesolid phasemicro extractions and gas chromatography mass spectrometry. First,the extracts from the branches and leaves of T. fargesii Franch. have different degrees of scavenging effects on DPPH,ABTS⁺,and hydroxyl radicals,among them,the branch extracts of T. fargesii Franch. in Kaizhou district had the highest scavenging rates of DPPH and ABTS⁺ free radicals,39.9% and 95.3% respectively;the leaf extracts of T. fargesii Franch. in Kaizhou district had the highest scavenging rates of hydroxy radical,reaching 69.8%. Second,the extracts from the branches and leaves of T. fargesii Franch. significantly inhibited the growth and reproduction of Escherichia coli,Bacillus subtilis and Staphylococcus aureus. Among them,the branch extracts of T. fargesii Franch. in Kaizhou district had the strongest inhibitory activity against B. subtilis,with the diameter of inhibition zone reaching 26.9 mm. Third,> 98% of the volatile components from the branches and leaves of T. fargesii Franch. Were terpenes,mainly monoterpenes and sesquiterpenes. In conclusion,the extracts of branches and leaves of T. fargesii Franch. have strong antioxidant and antibacterial activities,the main components of volatile components are monoterpene and sesquiterpene.

Key words: Torreya fargesii Franch., extract, anti-oxidant activity, antibacterial activity, volatile constituent

WANG Xiao-he, GU Xi-rong, QI Shun-ju, LI Jie, CUI Yao, LI De-xia, YANG Li-hui. Antioxidant Activity,Antibacterial Activity and Volatile Components of Extracts from the Branches and Leaves of Torreya fargesii Franch.[J]. Biotechnology Bulletin, 2021, 37(8): 152-161.

Figures/Tables 6

References 44

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化学成分 Chemical components	分子式 Molecular formula	分子量 Molecular weight	枝含量 Content of branches/%	叶含量 Content of leaves/%
α-蒎烯 α-pinene	C₁₀H₁₆	136	43.17	40.45
2-亚甲基-6,6-二甲基-[3.1.1]-二环庚烷 Bicyclo[3.1.1]heptane,6,6-dimethyl-2-methylene	C₁₀H₁₆	136	—	1.77
β-月桂烯 β-Myrcene	C₁₀H₁₆	136	1.94	1.49
2-亚甲基-7,7-二甲基-二环[2.2.1]庚烷 Bicyclo[2.2.1]heptane,7,7-dimethyl-2-methylene	C₁₀H₁₆	136	—	0.65
1,4-二甲基-4-乙烯基-环己烯 Cyclohexene,4-ethenyl-1,4-dimethyl	C₁₀H₁₆	136	2.45	—
β-水芹烯β-Phellandrene	C₁₀H₁₆	136	0.79	—
1,7,7-三甲基-二环庚醇乙酸酯 Bicyclo[2.2.1]heptan-2-ol,1,7,7-trimethyl,acetate	C₁₂H₂₀O₂	196	—	1.6
乙酸异龙脑酯Isobornyl acetate	C₁₂H₂₀O₂	196	0.86	—
石竹烯 Caryophyllene	C₁₅H₂₄	204	0.93	7.02
α-石竹烯 α-Caryophyllene	C₁₅H₂₄	204	6.03	6.8
α-甜没药烯 α-Bisabolene	C₁₅H₂₄	204	13.13	1.44
杜松萜烯 Cadinene	C₁₅H₂₄	204	13.18	10.05
α-摩勒烯 α-Muurolene	C₁₅H₂₄	204	0.84	0.77
α-荜澄茄油烯 α-Cubebene	C₁₅H₂₄	204	—	1.28
5-亚甲基-1-甲基-8-（1-甲基乙基）-1,6-环癸二烯 1,6-Cyclodecadiene,1-methyl-5-methylene-8-（1-methylethyl）	C₁₅H₂₄	204	16.68	26.68

化学成分 Chemical components	分子式 Molecular formula	分子量 Molecular weight	枝含量 Content of branches/%	叶含量 Content of leaves/%
α-蒎烯 α-pinene	C₁₀H₁₆	136	43.17	40.45
2-亚甲基-6,6-二甲基-[3.1.1]-二环庚烷 Bicyclo[3.1.1]heptane,6,6-dimethyl-2-methylene	C₁₀H₁₆	136	—	1.77
β-月桂烯 β-Myrcene	C₁₀H₁₆	136	1.94	1.49
2-亚甲基-7,7-二甲基-二环[2.2.1]庚烷 Bicyclo[2.2.1]heptane,7,7-dimethyl-2-methylene	C₁₀H₁₆	136	—	0.65
1,4-二甲基-4-乙烯基-环己烯 Cyclohexene,4-ethenyl-1,4-dimethyl	C₁₀H₁₆	136	2.45	—
β-水芹烯β-Phellandrene	C₁₀H₁₆	136	0.79	—
1,7,7-三甲基-二环庚醇乙酸酯 Bicyclo[2.2.1]heptan-2-ol,1,7,7-trimethyl,acetate	C₁₂H₂₀O₂	196	—	1.6
乙酸异龙脑酯Isobornyl acetate	C₁₂H₂₀O₂	196	0.86	—
石竹烯 Caryophyllene	C₁₅H₂₄	204	0.93	7.02
α-石竹烯 α-Caryophyllene	C₁₅H₂₄	204	6.03	6.8
α-甜没药烯 α-Bisabolene	C₁₅H₂₄	204	13.13	1.44
杜松萜烯 Cadinene	C₁₅H₂₄	204	13.18	10.05
α-摩勒烯 α-Muurolene	C₁₅H₂₄	204	0.84	0.77
α-荜澄茄油烯 α-Cubebene	C₁₅H₂₄	204	—	1.28
5-亚甲基-1-甲基-8-（1-甲基乙基）-1,6-环癸二烯 1,6-Cyclodecadiene,1-methyl-5-methylene-8-（1-methylethyl）	C₁₅H₂₄	204	16.68	26.68