Cloning and Prokaryotic Expression of p38β MAPK from Lutjanus sanguineus

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.030

Abstract

Abstract: Humphead snapper(Lutjanus sanguineus)p38β MAPK(Ls-p38β)was cloned using RACE method. The GenBank accession number is KJ502277. The full-length cDNA of p38β MAPK was 1 628 bp containing an open reading frame of 1 086 bp, encoding 361 amino acids with an estimated molecular weight of 41.6 kD and a theoretical pI of 5.74. Amino acid alignment indicated that Ls-p38β possessed a Thr-Gly-Tyr(TGY)dual phosphorylation motif of p38 MAPK family and shared 97% similarity with Oreochromis niloticus p38β. The prokaryotic expression vector pET-p38β was constructed and then transformed into BL21(DE3). SDS-PAGE and Western blotting analysis indicated that the recombinant protein of Ls-p38β was successfully expressed and molecular weight of expressed fusion protein was 60 kD.

Key words: Lutjanus sanguineus, p38β, MAPK, Prokaryotic expression

Xia Hongli,Cai Jia,Lu Yishan, Jian Jichang, Wu Zaohe. Cloning and Prokaryotic Expression of p38β MAPK from Lutjanus sanguineus[J]. Biotechnology Bulletin, 2014, 0(12): 177-183.

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