Study on Synthesis of Ethyl Butyrate by Butyric Acid Fermentation Broth Extraction Coupling with Esterification

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.035

Abstract

Abstract: Ethyl butyrate is one of the short chain ester, which is widely used in food, flavor, chemical, medicine and energy industry. In order to synthesize ethyl butyrate using butyric acid fermentation broth, butyric acid extraction from fermentation broth and estrification catalyzed by Novozym 435 in the extractive phase was studied. Tri-n-octylamine(TOA)+ cyclohexane were adopted as extractive agent and esterification reaction medium. Under the optimum conditions, the esterification reaction in the extractive phase had approached equilibrium in 5 h, the corresponding esterification yield was 81.42% and productivity was 65.96 mmol/(L?h). Good operational stability of Novozym 435 in this reaction system was observed. Ethyl butyrate yield relative to butyric acid was 0.65 mol/mol, which ethyl butyrate was synthesized by 100 mL butyric acid fermentation broth extraction-esterification coupling. These results suggest that synthesis of ethyl butyrate by butyric acid fermentation broth extraction-esterification coupling could realize the scale-up industrial production.

Key words: Ethyl butyrate, Butyric acid fermentation broth, Extraction, Esterification

Zhang Shexi, Chen Shaopei, Yang Jing, Wang Jufang. Study on Synthesis of Ethyl Butyrate by Butyric Acid Fermentation Broth Extraction Coupling with Esterification[J]. Biotechnology Bulletin, 2014, 0(12): 207-213.

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