Effect of Cell Density and Nutrition Supply on Cell-specific Virus Yields of Influenza Virus H1N1

doi:10.13560/j.cnki.biotech.bull.1985.2015.01.031

Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (1): 203-208.doi: 10.13560/j.cnki.biotech.bull.1985.2015.01.031

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Effect of Cell Density and Nutrition Supply on Cell-specific Virus Yields of Influenza Virus H1N1

Kong Wengang, Huang Ding, Luo Jian², Zhou Linting², Chen Ze², Liu Xuping, Tan Wensong, Deng Xiancun ^1，3

（1. State Key Laboratory of Bioreactor Engineering，East China University of Science and Technology，Shanghai 200237；2. Shanghai Institute of Biological Products，Shanghai 200052；3. Zhejiang Hisun Pharmaceutical（Hangzhou）Co.Ltd，Hangzhou 311404）

Received:2014-10-28 Online:2015-01-09 Published:2015-01-10

Abstract

Abstract: It was to investigate the phenomenon that high cell density with low cell-specific virus yields and find out the solution. Methods：High cell density was achieved by increasing the microcarrier concentration and enhancing nutrition supply, then the effect of cell concentration at infection（CCI）and virus maintenance medium（VMM）on virus titer and cell-specific virus yield（Svy）was studied. Results：The maximum cell density was up to 1.47×10⁷cells/mL in 12.6 g/L microcarrier culture using medium exchange strategy. Under high CCI（1×10⁷cells/mL）condition, the Svy was up to 5.14×10³ virions/cell by choosing appropriate virus maintenance medium, which is nearly twice of Svy in DMEM. Conclusion：In order to achieve higher influenza virus titer, culture conditions and nutrition demands at different stages of influenza production should be taken into consideration. The results in this work provide guidance for further development of industrial-scale vaccine production processes.

Key words: high-density culture, MDCK, influenza production, virions

Kong Wengang, Huang Ding, Luo Jian, Zhou Linting, Chen Ze, Liu Xuping, Tan Wensong, Deng Xiancun. Effect of Cell Density and Nutrition Supply on Cell-specific Virus Yields of Influenza Virus H1N1[J]. Biotechnology Bulletin, 2015, 31(1): 203-208.

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Effect of Cell Density and Nutrition Supply on Cell-specific Virus Yields of Influenza Virus H1N1

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