Effects of TOI on H1N1 Virus Propagation in MDCK Cell Culture

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.032

Abstract

Abstract: In order to improve the production efficiency of influenza vaccine in processes based on MDCK cell culture, the effects of TOI on influenza A(H1N1)virus propagation in MDCK cells were investigated. By infecting MDCK cells with H1N1 influenza virus at different TOI, the characteristics of MDCK growth and metabolism and influenza A(H1N1)virus were studied. The results showed that maximum influenza A(H1N1)virus yield reached when TOI was 72 h. However, the cell specific virus yield was reduced as TOI increased. To find out the reason behind this phenomenon, further analysis of TOI and CCI effects on cell specific virus yield were studied. Results showed that, the phenomenon was caused by cell status rather than high cell density. The study reveals the importance of cell status difference due to TOI, showed that influenza A(H1N1)virus production efficiency in MDCK cells could be improved by cell state adjusting.

Key words: TOI, influenza virus, MDCK cells

Huang Ding, Liu Xuping, Fan Li, Zhao Liang, Tan Wensong, Chen Ze. Effects of TOI on H1N1 Virus Propagation in MDCK Cell Culture[J]. Biotechnology Bulletin, 2015, 31(11): 243-250.

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