Cloning and Characterization Analysis of ZmJAZ4，a JAZ Family Gene in Maize(Zea mays L.)

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.013

Abstract

Abstract: Jasmonate ZIM-domain(JAZ)proteins are a group of plant-specific transcription factors, which have important function in plant growing development and abiotic stress by inhibiting the expression of genes associated with JA regulation. In this study, a novel JAZ family gene, named ZmJAZ4, was isolated from maize(Zea mays L.)inbred lines B73. The ZmJAZ4 cDNA has a total length of 651 bp and encodes a protein of 216 amino acids with a molecular weight of about 23.1 kD and a isoelectric points of 10.78, belonging to the basic protein. Real-time RT-PCR showed that ZmJAZ4 was mainly expressed in shoot apex meristem, tassel, developing seeds and endosperm. Phylogenetic analysis revealed that ZmJAZ4 was related to AtJAZ10 from Arabidopsis. Subcellular localization experiment showed that ZmJAZ4 was localized to the nucleus. ZmJAZ4 possessed no transcriptional activating activity in yeast cells. Various treatments were performed to detect the expression level of ZmJAZ4 in response to phytohormones or abiotic stresses. The transcripts of ZmJAZ4 was induced by PEG, NaCl, SA, GA and ABA treatment in shoots and that was induced by ABA and GA in roots. The above results showed that ZmJAZ4 may be an important transcriptional regulator, which participates in many hormones signaling pathways and abiotic stress.

Key words: Jasmonate, Zea mays Jasmonate ZIM-domain gene, Abiotic stress

Yan Shengwei, Sun Cheng, Zhou Xiaojin, Chen Rumei. Cloning and Characterization Analysis of ZmJAZ4，a JAZ Family Gene in Maize(Zea mays L.)[J]. Biotechnology Bulletin, 2015, 31(3): 96-101.

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