Screening，Identification and Optimization of Cellulase-producing Strains

doi:10.13560/j.cnki.biotech.bull.1985.2015.05.023

Abstract

Abstract: To isolate efficient cellulase-producing bacteria, 180 bacterial isolated from rotten wood, humus, and other soil samples, and 44 were screened from them and confirmed as cellulase-producing bacteria by Gram’s iodine solution staining. In the subsequent secondary screening tests, those 44 isolates were cultured in fermentation media and their total cellulase activities（FPase）were measured and compared. The strain J1-3-1, with the highest FPase activity, was identified as Sphingobacterium sp. by 16S rRNA sequence analysis. The optimal fermentation conditions for enzymes production of J1-3-1 were determined. Under the optimal conditions, its activities of FPase, CMCase, and β-glucosidase reached 8.76, 28.04和7.02 U/mL, respectively. The results demonstrated that J1-3-1 was a promising candidate for potential industrial production of cellulase.

Key words: cellulase-producing bacteria, screening, identification, fermentation, Sphingobacterium sp.

Li Zhengming, Zhang, Juan, Deng Zhongyang, Lu Fan, Qin Wensheng. Screening，Identification and Optimization of Cellulase-producing Strains[J]. Biotechnology Bulletin, 2015, 31(5): 146-152.

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