Principle and Application of Microscale Thermophoresis in Studies of Biomolecular Interactions

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.010

Abstract

Abstract: Microscale Thermophoresis(MST)is a new technique to study biomolecular interactions in recent years. It is based on thermophoresis, i.e., the directional movements of molecules in a temperature gradient, which results in subsequent changes of molecular properties such as sizes, charges, hydration shell and conformation. MST combines the precise fluorescence detection and sensitive thermophoresis and provides a sensitive, fast and precise detection technique to analyze biomolecular interactions. Working principle, detection process and application of MST in biology researches are reviewed here.

Key words: microscale thermophoresis, interaction analysis, binding studies, biomolecular interaction

Ai Qiushi, Cao Xiangyu, Zhao Qian, Niu Yali, Song Shuishan,. Principle and Application of Microscale Thermophoresis in Studies of Biomolecular Interactions[J]. Biotechnology Bulletin, 2015, 31(6): 67-73.

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