Preliminarily Research on Involvement of Arabidopsis GCR2 Responsing to N-Butyryl-DL-homoserine Lactone

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.014

Abstract

Abstract: N-Butyryl-DL-homoserine lactone（C4-HSL）is a main quorum sensing signal in gram-negative bacteria. It could significantly promote root elongation and activate Ca²⁺ channel at cytomembrane. C4-HSL can regulate metabolization of plant. However, little is known about the molecular mechanism of plants responding to C4-HSL. Arabidopsis thaliana GCR2 is receptor of abscisic acid（ABA）. It is important for metabolization of plant. This research aimed to explore whether the GCR2 is involved in the process of Arabidopsis thaliana reacting to C4-HSL. qRT-PCR showed that C4-HSL could regulate expression of GCR2. Expression of GCR2 was significantly upregulated after 1 h treated by C4-HSL and maximaized at 6 h. ELISA also showed that expression of GCR2 maximaized at 6 h. GCR2 was purified and condensed to 0.6 mg/mL for Microscale Thermophoresis（MST）measurement. MST indicated that dissociation constant（K_d）of GCR2 and C4-HSL was 166 nmol/L, which meant that they had strong binding affinity. Taking BSA as negative control, this certified that binding of GCR2 and C4-HSL had special character. These results showed that Arabidopsis GCR2 maybe involved in the pathway of plant responding to C4-HSL.

Key words: quorum sensing, N-acyl-homoserine lactones, Arabidopsis thaliana, microscale thermophoresis

Ai Qiushi, Zhang Zhe, Qu Lingbo, Liu Fang, Zhao Qian, Song Shuishan. Preliminarily Research on Involvement of Arabidopsis GCR2 Responsing to N-Butyryl-DL-homoserine Lactone[J]. Biotechnology Bulletin, 2015, 31(8): 94-101.

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