Dynamic Expression of miR169o and Its Target Genes OsNF-YAs in the Early Response to Water Deficiency in Rice

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.034

Abstract

Abstract: MicroRNAs（miRNAs）is noncoding RNAs and play important roles in plant development and response to various environment stresses. Recent evidence have indicated that miR169 is upregulated response to drought stress, and overexpression of miR169 contributes to improve the tolerance to drought stress in plant. However, it is still unclear to the expression pattern of miR169 under drought stress and the regulatory mechanism response to drought stress in rice. In this study, the dynamic expression patterns of miR169o and its target gene NF-YAs（Nuclear Factor Y A subunit, NF-YA）in the root, stem and leaf of rice were analysed systematically by qRT-PCR after water deficiency treatment. Generally, miR169o showed up-regulated after water deficiency stress treatment compared to that before treatment, while target gene NF-YAs showed imperfect reverse expression pattern to miR169o. In addition, the expression pattern and abundance of miR169o in rice root, stem and leaf suggested that the expression of miR169o is tissue-specific.

Key words: miR169o, OsNF-YAs, rice, water deficiency, expression pattern

Chen Yutong, Chen Huamin, Yu Chao, Amy Thein, Tian Fang, He Chenyang. Dynamic Expression of miR169o and Its Target Genes OsNF-YAs in the Early Response to Water Deficiency in Rice[J]. Biotechnology Bulletin, 2015, 31(8): 76-81.

References

[1] Rogers K, Chen X. Biogenesis, turnover, and mode of action of plant microRNAs[J]. Plant Cell, 2013, 25（7）：2383-2399.
[2] Bartel DP. MicroRNAs：genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116（2）：281-297.
[3] Jones-Rhoades MW, Bartel DP, et al. MicroRNAs and their regula-tory roles in plants[J]. Annu Rev Plant Biol, 2006, 57：19-53.
[4] Sunkar R, Chinnusamy V, Zhu J, et al. Small RNAs as big players in plant abiotic stress responses and nutrient deprivation[J]. Trends in Plant Science, 2007, 12（7）：301-309.
[5] Khraiwesh B, Zhu JK, Zhu J. Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants[J]. Biochim Biophys Acta, 2012, 1819（2）：137-48.
[6] Covarrubias AA, Reyes JL. Post-transcriptional gene regulation of salinity and drought responses by plant microRNAs[J]. Plant Cell Environ, 2010, 33（4）：481-489.
[7] Zhao M, Ding H, Zhu JK, et al. Involvement of miR169 in the nitrogen-starvation responses in Arabidopsis[J]. New Phytol, 2011, 190（4）：906-915.
[8] Lundmark M, Korner CJ, Nielsen TH. Global analysis of microRNA in Arabidopsis in response to phosphate starvation as studied by locked nucleic acid-based microarrays[J]. Physiol Plant, 2010, 140（1）：57-68.
[9] Li WX, Oono Y, et al. The Arabidopsis NFYA5 transcription factor is regulated transcriptionally and posttranscriptionally to promote drought resistance[J]. Plant Cell, 2008, 20（8）：2238-2251.
[10] Ni Z, Hu Z, Jiang Q, et al. GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress[J]. Plant Mol Biol, 2013, 82（1-2）：113-129.
[11] Liu HH, Tian X, Li YJ, et al. Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana[J]. RNA, 2008, 14（5）：836-843.
[12] Xu MY, Zhang L, Li WW, et al. Stress-induced early flowering is mediated by miR169 in Arabidopsis thaliana[J]. J Exp Bot, 2014, 65（1）：89-101.
[13] Sorin C, Declerck M, Christ A, et al. A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis[J]. New Phytol, 2014, 202（4）：1197-1211.
[14] Liu HJ, Chen LG, Zhu PP, et al. Effect of hyacinth mulching on rice（Oryza sativa L. ）uptake and utilization of nitrogen[J]. Environmental Science, 2011, 32（5）：1292-1298.
[15] 潘雅姣, 傅彬英, 王迪, 等. 水稻干旱胁迫诱导DNA甲基化时空变化特征分析[J]. 中国农业科学, 2009, 42：3009-3018.
[16] Zhao B, Ge L, Liang R, et al. Members of miR-169 family are induced by high salinity and transiently inhibit the NF-YA transcription factor[J]. BMC Mol Biol, 2009, 10：29.
[17] Hoagland DR, Arnon DI. The water-culture method for growing plants without soil[M]. 2nd ed. Circular. California Agricultural Experiment Station, 1950：347.
[18] Rio DC, Ares M, Hannon GJ, et al. Purification of RNA using TRIzol（TRI reagent）[J]. Cold Spring Harbor Protocols, 2010（6）：pdb. prot5439.
[19] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using Real-Time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25（4）：402-408.
[20] Yang B, Ma HY, Wang XM, et al. Improvement of nitrogen accumulation and metabolism in rice（Oryza sativa L. ）by the endophyte Phomopsis liquidambari[J]. Plant Physiol Biochem, 2014, 82：172-182.
[21] Liu Q, Zhang YC, Wang CY, et al. Expression analysis of phytohormone-regulated microRNAs in rice, implying their regulation roles in plant hormone signaling[J]. FEBS Letters, 2009, 583（4）：723-728.
[22] Jia X, Wang WX, Ren L, et al. Differential and dynamic regulation of miR398 in response to ABA and salt stress in Populus tremula and Arabidopsis thaliana[J]. Plant Molecular Biology, 2009, 71（1-2）：51-59.
[23] Zhao B, Liang R, Ge L, et al. Identification of drought-induced microRNAs in rice[J]. Biochem Biophys Res Commun, 2007, 354（2）：585-590.
[24] Jia W, Wang Y, et al. Salt-stress-induced ABA accumulation is more sensitively triggered in roots than in shoots[J]. Journal of Experimental Botany, 2002, 53（378）：2201-2206.
[25] Daszkowska-Golec A, Szarejko I. The molecular basis of ABA-mediated plant response to drought[J]. Agricultwre and Biological Scieurs, 2013：103-133.
[26] Wilkinson S, Davies WJ. ABA-based chemical signalling：the co-ordination of responses to stress in plants[J]. Plant, Cell & Environment, 2002, 25（2）：195-210.
[27] Chaves MM, Maroco JP, Pereira JS. Understanding plant responses to drought—from genes to the whole plant[J]. Functional Plant Biology, 2003, 30（3）：239-264.