Cloning and Expression Analysis of PtLIR1 Gene from Puccinellia tenuiflora Under NaCl Stress

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.022

Abstract

Abstract: Light regulated protein 1-like gene is closely associated with plant stress resistance and may mediated sucrose level. In this study, the open reading frame of PtLIR1 gene was cloned from Puccinellia tenuiflora, and its expression characteristics under NaCl stress were analyzed. The full length open reading frame of PtLIR1 was 408 bp, which encoded 135 amino acids. The deduced amino acid sequence of PtLIR1 gene shared high identity with its homologs from Oryza sativa, Zea mays, Brassica rapa, Thellungiella halophila, Arabidopsis thalian, Oryza brachyantha, Setaria italica, Solanum tuberosum, Vitis vinifera, Brachypodium distachyon, Glycine max, respectively, and showed the highest identity of 80% with Lolium perenne. At the early stage of salt stress, the expression of PtLIR1 was negatively correlated with the accumulation of soluble sugar, and the other sucrose metabolism related genes may play more important roles in the regulation of sucrose level. However, during the late stage of salt stress, PtLIR1 gene was up-regulated in Puccinellia tenuiflora roots and may play a vital role in regulating sucrose level. PtLIR1 gene was involved in response to NaCl stress, and maybe contribute to the salt tolerance of Puccinellia tenuiflora.

Key words: Puccinellia tenuiflora, light regulated protein 1-like protein, PtLIR1 gene, root, salt stress

Zhou Qi, Luo Chun, Guo Min, Fu Chang. Cloning and Expression Analysis of PtLIR1 Gene from Puccinellia tenuiflora Under NaCl Stress[J]. Biotechnology Bulletin, 2015, 31(11): 166-172.

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