Research Advance on the Structure, Molecular Modification, and Fermentation of Lipoxygenases

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.005

Abstract

Abstract: Lipoxygenases(EC1.13.11.12)catalyze and oxidize the polyunsaturated fatty acids containing Z, Z-1, 4-pentadiene structures to form the conjugated hydroperoxides of fatty acid, and they are widely used in food industry, chemical industry and pharmaceutical industry. Since lipoxygenase was firstly discovered in soybean in 1932, it has been detected in many animal and vegetable tissues as well as microorganisms. Broad sources led to various structure types of lipoxygenase, including classic, fusing, Β-sheet deleted, and Mn²⁺-containing structures. To improve the application performance, molecular modification techniques, such as site-directed mutagenesis and fusing with self-assembling amphiphilic peptides, have been used to enhance the specific activity and thermal stability of lipoxygenase. Considering the natural advantages of fermentation method, the construction of recombinant strains producing high-yield lipoxygenase has become the research hotspot recently. The advances on the structure, molecular modification, and fermentation of classic lipoxygenases were briefly summarized for providing a reference for further studies.

Key words: lipoxygenase, structure and function, molecular modification, fermentation

Liu Song, Lu Xinyao, Zhou Jingwen, Du Guocheng, Chen Jian. Research Advance on the Structure, Molecular Modification, and Fermentation of Lipoxygenases[J]. Biotechnology Bulletin, 2015, 31(12): 34-41.

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