Research Progress Regarding to Large-scale Packaging System of rAAV

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.009

Abstract

Abstract: As the only gene therapy vector certificated by EU FDA, recombinant adeno-associated virus(rAAV)has advantages of wide host range, high transfection efficiency, non-pathogenic, low immunogenicity and long-term expression of transgenosis. With the expansion of applying rAAV for gene therapy clinical trials, the shortage of capacity of packing system in traditional rAAV has emerged, thus the rAAV packaging system that can be easily enlarged and scaled up to solve the existing problem of supply and demand is in urgent need. Therefore, on the basis of describing traditional rAAV packaging system, the focus of this review is to emphatically expound the baculovirus insect cell lines, yeast, and vaccinia virus-adenovirus method, especially with more expectation to the last one. The aim of this article is to introduce several preferable systems for scale packaging of rAAV, and discuss the trend of development.

Key words: rAAV, gene therapy, vector, packaging system

Zhan Shenbiao, Tang Mingqing, Gan Na, Cao Yuanqing, Li Zhaofa. Research Progress Regarding to Large-scale Packaging System of rAAV[J]. Biotechnology Bulletin, 2015, 31(12): 63-69.

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