The Effects of Ethanol Extract from Aspongopus chinensis on the Activities of Antioxidant Enzymes in Skeletal Muscle of Exercised Rats and Their Gene Expression Levels

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.021

Abstract

Abstract:

The effects of ethanol extract from Aspongopus chinensis(EEAC)on the activities of antioxidant enzymes in skeletal muscle of exercised rat and their gene expression levels were analyzed in this study. The EEAC was supplemented to rats that were given heavy-load swimming training at 3 dose levels of 0.5 g/kg, 1.0 g/kg and 1.5 g/kg based on the average weight of each group. The activities and encoded genes expression levels of antioxidant enzymes in skeletal muscle were measured after 8 weeks training. The activities of SOD, CAT and GST with EEAC supplementation were increased significantly compared to non-supplemented training rats, and the encoded genes for these 3 enzymes were up-regulated also. The results suggest that the one mechanism for the increase of antioxidant enzymes activities in skeletal muscle of exercised rats is that the expression levels of encoded genes were up-regulated by EEAC supplementation.

Key words: ethanol extract from Aspongopus chinensis, exercised rat, antioxidant enzymes in skeletal muscle, expression level, enzyme activity

Gao Yinghui, Zhou Wanhong, Dou Peng, Qi Yiman, Wang Dun. The Effects of Ethanol Extract from Aspongopus chinensis on the Activities of Antioxidant Enzymes in Skeletal Muscle of Exercised Rats and Their Gene Expression Levels[J]. Biotechnology Bulletin, 2015, 31(12): 146-149.

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