A Prokaryotic Expression of Recombinant IFNγ from Takifugu rubripes

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.025

Abstract

Abstract: The aim is to provide a simple and efficient method to acquire recombinant fusion proteins of IFNγ of T. rubripes. In this study, total RNA was extracted from the kidney tissue of T. rubripes, and used as a template to amplify gene sequences of interferon γ(IFNγ)by RT-PCR. The recombinant DNA pET-32a-IFNγ was constructed by ligating the mature peptide sequences of IFNγ and prokaryotic expression vector pET-32a(+). The genetic engineering bacteria of recombinant expressed IFNγ were obtained by transforming pET-32a-IFNγ into the competent cells of Escherichia coli BL21(DE3). The fusion proteins were obtained under the induction of IPTG. By purifying the express products and Western blotting test, the results showed the high-efficiency expression of recombinant pET-32a-IFNγ in E. coli and the accurate target protein.

Key words: Takifugu rubripes, interferon γ, prokaryotic expression

Ma Pu, Sun Saihong, Wang Yufang, Li Hui, Liu Haiying, Jiang Zhiqiang, Qiu Xuemei, Liu Yang, Zhang Tao, Wang Xiuli. A Prokaryotic Expression of Recombinant IFNγ from Takifugu rubripes[J]. Biotechnology Bulletin, 2015, 31(12): 174-179.

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