Abstract: Living organisms undergo a series of stresses from abiotic environment, and these stresses will affect the changes of genetic transcription and the expression of protein for quick adaptation to the changing environment. Two-dimensional electrophoresis and mass spectrometry were used to investigate the effects of high-temperature stresses on the proteome of cell wall in Saccharomyces cerevisiae. The results showed that, in S. cerevisiae FFC2146 under high temperature, proteins of cell walls had new Ssa2 and small molecules guanosine triphosphatase, inorganic pyrophosphatase was up-regulated, pyruvate kinase was deficient or even disappeared, and the expression of glucose-6-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase was down-regulated. These results indicated that the heat shock protein Ssa2 protected cell walls to be intact at a high temperature, therefore the cells grew and reproduced continuously；EMP glycolytic pathway of S. cerevisiae under heat stress was blocked, glycolytic pathway turned HMP pathway by transketolase, enough energy was obtained to maintain normal metabolism of cell.

Key words: Saccharomyces cerevisiae, two-dimensional electrophoresis, heat shock protein