Abstract: In order to investigate the molecular mechanism of high temperature stress in Mizuhopecten yessoensis, as well to find effective ways to solve the problem of summer mortality. The HSP60（heat shock protein 60）as a member of the highly conserved heat shock protein family, not only can help other protein fold correctly and restore the natural conformation in the condition of stress, but also can be used as a danger signal to the natural immune system. Specific primer was designed based on transcriptome sequence of HSP60, and the full sequence of cDNA of heat shock protein 60（MyHSP60）from Japanese scallop Mizuhopecten yessoensis was cloned by RACE. The cDNA sequence was 3 368 bp in length, including 68 bp 5' UTR, 1 569 bp 3' UTR and 1 731 bp open reading frame encoding 576 amino acids. Using gene walking the promoter region of MyHSP60 gene sequences was amplified and sequenced, the sequences of promoter region of MyHSP60 gene was 1 236 bp, which contained 2 TATA boxes, 4 CCAAT boxes, and 3 heat shock elements. The phylogenetic tree based on amino acid sequence matched with the traditional species trees based on basic anastomosis. The Real-time PCR result showed that HSP60 in all tissues of M. yessoensis was expressed, the expression in the tissues after heating increased extremely significantly in kidney, and significantly in hepatopancreas, mantle, hemolymph and muscle（P<0.05）, indicating that the gene plays a certain role in the process of heat stress.

Key words: Mizuhopecten yessoensis, heat shock protein 60, cloning, promoter sequence, expression characterization