Timeliness of Four Bacillus Strains Against Botrytis cinerea of Garlic Sprouts

doi:10.13560/j.cnki.biotech.bull.1985.2016-1216

Abstract

Abstract: The aim of this work is to identify the four strains of Bacillus at the species level and study their inhibitory effects on Botrytis cinerea in vitro and their timeliness of fresh-keeping garlic sprouts in vivo. The plate-to-plate method and the mycelial growth method were used for in vitro experiments,and the injury inoculation method was for prevention and therapy treatment in vivo experiments in different periods. Results showed that four Bacillus strains in vitro presented significant inhibitory effects on colonization and mycelial growth of Botrytis cinerea in contrast to the control,and in the order of HB-2 > B579 > B1> B001（P < 0.05）. In vivo experiments under 20℃ showed that preventive treatments with four Bacillus strains effectively controlled the expansion of B. cinerea lesion,and the effect was better than that of therapy treatment,but the optimal preventive time of various Bacillus varied. Among them,the preventive treatment with Bacillus HB-2 and B579 better maintained the soluble solids,vitamin C and titratable acid content of garlic sprouts. In conclusion,Bacillus HB-2 is more effective than the other three strains of Bacillus to prevent garlic mold of garlic sprouts.

Key words: Bacillus, Botrytis cinerea, garlic sprouts fresh-keeping, timeliness

WEI Qian, ZHANG Na, ZHANG Ping, LI Ming-gang, YAN Rui-xiang. Timeliness of Four Bacillus Strains Against Botrytis cinerea of Garlic Sprouts[J]. Biotechnology Bulletin, 2017, 33(6): 112-120.

References

[1] 王向鹤, 裴占江, 王粟, 等. CO 2 气肥对北方棚室蒜薹生长发育及品质的影响[J]. 湖北农业科学, 2015, 54（12）：2919-2923, 2953.
[2] 薛婷, 陈丽, 李喜宏, 等. 蒜薹采后病原真菌鉴定及侵染特点的研究[J]. 安徽农业科学, 2006, 34（3）：513-515.
[3] 刘丽影. 影响蒜薹物流质量安全的病害诊断及致病性研究[D]. 上海：华东师范大学, 2013.
[4] 赵淑艳, 陈丽, 李喜宏, 等. 蒜薹采后病原菌种类及侵染规律研究[J]. 中国农学通报, 2005, 21（9）：74-78.
[5] 薛婷. 蒜薹采后病害与生防拮抗菌的筛选研究[D]. 天津：天津科技大学, 2006.
[6] Turner JT, Backman PA. Factors relating to peanut yield increases after seed treatment with Bacillus subtilis[J]. Plant Disease, 1991, 75（4）：347-353.
[7] 赵新林, 赵思峰. 枯草芽孢杆菌对植物病害生物防治的作用机理[J]. 湖北农业科学, 2011, 50（15）：3025-3028.
[8] 刘雪, 穆常青, 蒋细良, 等. 枯草芽孢杆菌代谢物质的研究进展及其在植病生防中的应用[J]. 中国生物防治, 2006, 22（增刊）：179-184.
[9] 穆常青. 枯草芽孢杆菌B-332菌株及其抗菌物质对稻瘟病的生防作用研究[D]. 北京：中国农业科学院, 2006：8-10.
[10] 赵妍, 邵兴锋, 屠康, 等. 枯草芽孢杆B10对采后草莓果实病害的抑制效果[J]. 果树学报, 2007, 24（3）：339-343.
[11] 伏波, 姚娟妮, 高小宁, 等. 植物内生枯草芽孢杆菌 Em7菌株对葡萄灰霉病菌的抑菌活性[J]. 农药学学报, 2016, 18（4）：465-471.
[12] 魏东盛, 陈云芳, 刘大群. 芽孢杆菌B21菌株及其发酵液对番茄灰霉菌C31的影响[J]. 河北农业大学学报, 2002, 25（4）：73 -79.
[13] 黄秀梨. 微生物学实验指导[M]. 北京：高等教育出版社, 1999.
[14] Hillis DM, Moritz CM, Mable BK. Molecular Systematics[M]. MasSachusetts：Sinauer Associates, 1990：411-501.
[15] 库尔班江, 赛丽曼. 碘量法测水果蔬菜中维生素C的含量[J]. 伊犁师范学院学报：自然科学版, 2007（3）：28-31.
[16] 李合生, 孙群, 赵世杰, 等. 植物生理生化实验原理与技术[M]. 北京：高等教育出版社, 2000：123-124, 195-197, 246-268.
[17] 黄曦, 许兰兰, 黄荣韶, 等. 枯草芽孢杆菌在抑制植物病原菌中的研究进展[J]. 生物技术通报, 2010, （1）：24-28.
[18] Liu Y, Chen Z, NGTB, et a1. Bacisubin, an antifungal protein with ribonuclease and hemagglutinating activities from Bacillussubtilis strainB-916[J]. Peptidkes, 2007（28）：553-559.
[19] 黄现青, 别小妹, 吕凤霞, 等. 枯草芽孢杆菌fmbJ产脂肽抑制点青霉效果及其桃防腐试验[J]. 农业工程学报, 2008, 24（1）：263-267.
[20] 周晓琳, 牟文良, 王庆国. 切除薹苞处理对蒜薹货架期品质的影响[J]. 保鲜与加工, 2012, 12（2）：20-23.
[21] 刘璐, 代红军, 王振平. 胶冻样芽孢杆菌对赤霞珠葡萄光合作用及果实品质的影响[J]. 农业科学研究, 2016, 37（2）：25-28.
[22] 肖嫩群, 黄晓辉, 胡汝晓, 等. 芽孢杆菌B13对白菜产量和品质的影响[J]. 中国微生态学杂志, 2014, 26（1）：27-30, 33.