Isolation and Identification of Endophytic Bacteria in Red Grape，and Screening of Antagonistic Bacteria Against Botrytis cinerea

doi:10.13560/j.cnki.biotech.bull.1985.2016.04.024

Abstract

Abstract:

This experiment aims to separate the endophytic bacteria，which have the strongly antagonistic effect on Botrytis cinerea，from different parts of red grape in Xinjiang. The tissue isolation was adopted to separate the endophytic bacteria from different parts of red grape，and concurrently 16S rDNA sequence determination and homology analysis were carried out. Meanwhile，the antagonistic effect of endophytic bacteria on B. cinerea was analyzed by confrontation culture method and disc diffusion assay method. The results showed that 5 of 18 strains of endophytic bacteria had solid antagonistic effect，they were NR4-1，NR5-1，NG4-1，NP1-1，and PG1 respectively，and the bacteriostasis rate reached 68.7%.

Key words: endophytic bacteria, Botrytis cinerea, antagonism, isolation, identification

ZHOU Ling-xuan,LIU Ya. Isolation and Identification of Endophytic Bacteria in Red Grape，and Screening of Antagonistic Bacteria Against Botrytis cinerea[J]. Biotechnology Bulletin, 2016, 32(4): 184-189.

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