It is the major way to produce hybrid seeds by cytoplasmic male sterile line because it does not need manual emasculation，thus not only the cost of breeding is reduced，but also the purity of hybrid seeds is ensured. In this paper，the developing period of male sterility，the differences of energies，substances and hormones between male sterile line and its maintaining line are reviewed. The mechanisms of male sterility and its restorer are summarized from the aspects of genetic analysis，quantitative trait loci and molecular biology.
