The Transformation of Drought-resistant Gene into Maize by Microprojectile Bombardment

doi:10.13560/j.cnki.biotech.bull.1985.2016.05.008

Abstract

Abstract: A drought-resistance function gene，VaP5CS，was cloned from mothbean（Vigna aconitifolia）. Plant expression vector pBPC-P5CS-F129A was constructed using gene engineering strategy，then the genetic transformation of young embryo callus in maize inbred line Jing 501 was performed by microprojectile bombardment. Further，the transformed explants were directly selected on the medium containing 10 mg/L glufosinate，and the glufosinate-resistant plantlets were confirmed by PCR amplification and Northern blot identification. Finally，the different drought resistance indexes of P5CS-transgenic maize plants were analyzed. The results showed that the transgenic plants presented the improved drought resistance comparing to control plants. Under drought stress，the proline content in the P5CS-transgenic maize plant was higher than that in the control，while the MDA content was lower than that in the control.

Key words: Zea mays, callus, microprojectile bombardment, glufosinate, genetic transformation, drought resistance

LI Zhi-liang,WU Zhong-yi,YANG Qing,ZHANG Xi-tai,YE Jia,XING Hao-chun,CHEN Jian-zhong,HUANG Cong-lin,. The Transformation of Drought-resistant Gene into Maize by Microprojectile Bombardment[J]. Biotechnology Bulletin, 2016, 32(5): 61-68.

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