Cloning，Expression and Bioinformatics Analysis of IQM5.2 from Arabidopsis

doi:10.13560/j.cnki.biotech.bull.1985.2016.05.009

Abstract

Abstract: The Arabidopsis IQM5，the fifth member of IQM family，is encoded by At5g57010. So far，there is only one splicing mode found for the IQM5 gene in the biological literatures and databases，i.e.，At5g57010.1（IQM5.1）. In this study，a new splicing mode for IQM5 gene was found by RT-PCR and T-A cloning method，designated as At5g57010.2（IQM5.2）. Bioinformatics methods and semi-quantitative and quantitative RT-PCR were employed to analyze the properties of proteins encoded by new CDS sequence，and the ratio of IQM5.1 and IQM5.2 in different organs，respectively. The results revealed that IQM5.2 encoded a polypeptide of 300 amino-acid residues，and its N-terminus shared sequence homology with the pea heavy metal-induced protein 6A（HMIP6A）. The protein retained an IQ motif and was a calmodulin-binding protein of the calcium independent. There were transcripts of IQM5.1 and IQM5.2 in the seedling，rosette leaf，cauline leaf，stem，and flower bud，moreover，the ratios of them in the different organs varied.

Key words: IQM5.2, alternative splicing, calmodulin-binding protein, gene expression, Arabidopsis

GONG Lu-ping,XIAO Wen-hui,ZHOU Yu-ping,HUANG Xiao-ling,TIAN Chang-en. Cloning，Expression and Bioinformatics Analysis of IQM5.2 from Arabidopsis[J]. Biotechnology Bulletin, 2016, 32(5): 69-74.

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