Transcriptome Analysis of Drought-resistant Genes in Hainan Shanlan Upland Rice

doi:10.13560/j.cnki.biotech.bull.1985.2020-0422

Abstract

Abstract:

Shanlan upland rice is an unique local upland rice in Hainan province,which is rich in drought- resistant gene resources. In this study,a Hainan Shanlan upland rice variety,Baishanuo was used to study the changes of transcriptome level after simulated drought stress. The main results were as follows：2 791 genes were differentially expressed after simulated drought stress,and 184 transcription factors were significantly up-regulated after simulated drought stress. These transcription factors include Znf,AP2/ERF,MYB,NAC,bZIP and so on,among which the number of Znf transcription factors was the largest. Expression analysis showed that some unannotated genes,such as Os01g0910800,their expressions increased with the time of stress,and the multiple of expression was obvious. The transcriptome data and digital gene expression profiling data obtained in this study provide information for the study of drought resistance mechanism of Shanlan upland rice,and lay a foundation for further analysis of the drought resistance mechanism of Shanlan upland rice.

Key words: Shanlan upland rice, upland rice, RNA-seq, drought resistance gene

ZHAI Nan-xin, CHI Hui, XIA Yue-lin, LIU Cai-yue, PEI Xin-wu, YUAN Qian-hua. Transcriptome Analysis of Drought-resistant Genes in Hainan Shanlan Upland Rice[J]. Biotechnology Bulletin, 2020, 36(12): 12-20.

Figures/Tables 10

References 24

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Gene_ID /Name	Forward primer（5'-3'）	Reverse primer（5'-3'）
OsLEA3-2	CCAAGAACAAGCTGGGCGAG	CTTGAACTCCGTCGCCTTCT
RAB21	GCTCCAGCTCAAGCTCGTC	CATGGCATGCTGCTGCTC
Os09g0351700	TCTCGAACAGCAACCTGCAT	GGATGGAATTGTCCCGCTCA
Os07g0658600	GGGTTCGACACGTGCTACA	GGTGTGGATCACCACGTTCT
Os02g0693700	GTTCTACGAGCCTCTCACGG	CTGGTTGACGAGGCCAATCT
Os11g0115100	TCAGGAACCTCAACATGGGC	CTTGGAGCAGTCGACGGAG
Os07g0251900	CTTCGCTCGGGAACCTAACA	TGAGGTAGAGTGACCGGAGG
Os01g0910800	TGGCGCAGTACTACTCGGA	GCCGGTGCAGGAGTCGTA
OsActin	TGGCATCTCTCAGCACATTCC	TGCACAATGGATGGGCCAGA

Gene_ID /Name	Forward primer（5'-3'）	Reverse primer（5'-3'）
OsLEA3-2	CCAAGAACAAGCTGGGCGAG	CTTGAACTCCGTCGCCTTCT
RAB21	GCTCCAGCTCAAGCTCGTC	CATGGCATGCTGCTGCTC
Os09g0351700	TCTCGAACAGCAACCTGCAT	GGATGGAATTGTCCCGCTCA
Os07g0658600	GGGTTCGACACGTGCTACA	GGTGTGGATCACCACGTTCT
Os02g0693700	GTTCTACGAGCCTCTCACGG	CTGGTTGACGAGGCCAATCT
Os11g0115100	TCAGGAACCTCAACATGGGC	CTTGGAGCAGTCGACGGAG
Os07g0251900	CTTCGCTCGGGAACCTAACA	TGAGGTAGAGTGACCGGAGG
Os01g0910800	TGGCGCAGTACTACTCGGA	GCCGGTGCAGGAGTCGTA
OsActin	TGGCATCTCTCAGCACATTCC	TGCACAATGGATGGGCCAGA

Sample name	Total reads	Total mapped	Multiple mapped	Uniquely mapped	Non-splice reads	Splice reads
No-drought	57 202 689	53 682 100 93.77%	1 407 400 2.45%	52 274 700 91.32%	33 494 592 58.51%	18 780 108 32.81%
Drought	51 734 760	48 915 870 94.56%	1 027 373 1.99%	47 888 497 92.57%	30 689 990 59.31%	17 198 507 33.27%

Sample name	Total reads	Total mapped	Multiple mapped	Uniquely mapped	Non-splice reads	Splice reads
No-drought	57 202 689	53 682 100 93.77%	1 407 400 2.45%	52 274 700 91.32%	33 494 592 58.51%	18 780 108 32.81%
Drought	51 734 760	48 915 870 94.56%	1 027 373 1.99%	47 888 497 92.57%	30 689 990 59.31%	17 198 507 33.27%

Baishanuo upland rice	Up	Down	Total
Known genes	1 586	1 064	2 650
Unknown Gene	83	58	141
Total	1 669	1 122	2 791