Cloning and Prokaryotic Expression of Interferon Regulatory Factor 2 from Takifugu rubripes

doi:10.13560/j.cnki.biotech.bull.1985.2016.05.014

Abstract

Abstract: Interferon Regulatory Factor（IRF）is a transcription factor with multifunctions. The full length cDNA of Takifugu rubripes interferon regulatory factor 2（IRF2）was cloned by RT-PCR and rapid amplification of cDNA ends（RACE）. The full-length cDNA of the gene was 1 552 bp，including a 187 bp 5' non-coding region，a 429 bp 3' non-coding region，and 936 bp coding region，and it encoded 311 amino acids. The recombinant pET32a（+）-IRF2 was constructed by ligating code sequence of IRF2 with prokaryotic expression vector pET32a（+）. Then，the recombinant genetic engineering bacteria with expressed IRF2 were obtained by transforming pET32a（+）-IRF2 into competent cell Escherichia coli BL21（DE3）. pET32a（+）-IRF2 was expressed in BL21 under the induction of IPTG. The results from the detection of the purified products by Western blotting showed that the expression of the IRF2 gene in E. coli was high，and the target protein was accurate.

Key words: Takifugu rubripes, interferon regulatory factor 2, rapid amplification of cDNA end, prokaryotic expression

SUN Sai-hong,MA Pu,SUN He,KONG De-rong,Li Hui,QIU Xue-mei,JIANG Zhi-qiang,LIU Hai-ying,LIU Yang. Cloning and Prokaryotic Expression of Interferon Regulatory Factor 2 from Takifugu rubripes[J]. Biotechnology Bulletin, 2016, 32(5): 107-113.

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