Effects of Different Temperature on Starch Metabolism in Different Growth Stages of Tobacco（Nicotiana tabacum）

doi:10.13560/j.cnki.biotech.bull.1985.2016.05.027

Abstract

Abstract: In order to clarify the effects of different temperatures on starch metabolism in different growth stages of tobacco，using Yunyan 87 as material growing in artificial climate chamber to simulate the different climates（Jiangchuan in Yunnan，Zunyi in Guizhou，and Xiang in Henan）in the stages of transplanting to resettling，resettling to squaring，and mature，we studied the effects of growth temperature on tobacco starch content and enzyme activity，and detected the expression changes of genes related to starch metabolism by RT-PCR. The results showed that，the starch content increased with the continuous growth of tobacco，and achieved the highest in the stage of mature. The temperature of Zunyi in Guizhou was beneficial to the accumulation of starch and the AGPase activity increased in the stage of transplanting to resettling. The temperature of Jiangchuan in Yunnan was conducive to the accumulation of starch content and the activities of AGPase and SSs raised in the stages of resettling to squaring and mature. The starch accumulation under the temperature of Xiang in Henan in any stage was little. The above results indicated that the temperature ultimately determined the formation of tobacco leave style by impacting the genes expressions of genes related to starch metabolism，thereby affecting the activities of related enzymes，as well as the metabolism and transport of starch.

Key words: tobacco, growth temperature, starch metabolism, Real-time PCR

ZHANG Jian-bo,JIN Yun-feng,WANG Sha-sha,YANG Hui-qin,PANG Tao,LI Jun-ying,CUI Ming-kun,GONG Ming,. Effects of Different Temperature on Starch Metabolism in Different Growth Stages of Tobacco（Nicotiana tabacum）[J]. Biotechnology Bulletin, 2016, 32(5): 200-211.

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