Research Progress on Recombinase Polymerase Amplification

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.008

Abstract

Abstract: Recombinase Polymerase Amplification(RPA)is a recently developed isothermal amplification method that offers highly sensitive and specific DNA and RNA detection. Using RPA，the initial copy number of target nucleic acid sequence from different samples can be absolutely quantified，and multiple target nucleic acid sequences can also be detected simultaneously. Combining with less complicated device such as lateral flow strips or a sequence-specific fluorescent probe，the results can be observed directly. This article summarized the fundamental principles，continuous development and technical features of RPA. In addition，research and application progress of RPA in the field of in vitro diagnostic，pathogen detection and so forth were reviewed，aiming at providing guidance for further development and application of this method.

Key words: recombinase polymerase amplification, nucleic acid isothermal amplification technologies, nucleic acid detection

JING Zhi-gang, DONG Hao, DI Dong-dong, TIAN Li-li, FAN Wei-xing. Research Progress on Recombinase Polymerase Amplification[J]. Biotechnology Bulletin, 2016, 32(6): 47-53.

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