Expression of diptericin Gene from Musca domestica in Escherichia coli

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.020

Abstract

Abstract: In order to efficiently acquire the antibacterial peptide by prokaryotic expression，diptericin gene for the antibacterial peptide in housefly was reversely synthesized by RT-PCR method. Then，diptericin gene was cloned to pGEX-4T-1 expression vector and transformed into Escherichia coli BL21 for protein expression. Sequencing result showed that housefly’s diptericin gene was 345 nucleotides in length. Recombinant pGEX-4T-1 was induced by IPTG，and the expression of diptericin fusion protein was detected by SDS-PAGE electrophoresis and Western blot. The result showed that diptericin fusion protein with GST-tag was about 37 kD，and the target protein was purified by GST purification column.

Key words: housefly, antibacterial peptide, diptericin, prokaryotic expression

LU Min, BAI Jie, WEI Feng-xian, WANG Lin-yi, XU Bin, YIN Qing-qiang, LI Shao-yu. Expression of diptericin Gene from Musca domestica in Escherichia coli[J]. Biotechnology Bulletin, 2016, 32(6): 143-147.

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