Research Progress on Genetic Engineering for Long-chain Polyunsaturated Fatty Acids EPA and DHA

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.001

Abstract

Abstract:

Long-chain polyunsaturated fatty acids（LCPUFAs）play an important role in maintaining human health. The demand of edible EPA and DHA has significantly increased in recent years. However,the fish oil resource is obviously reduced due to the environmental pollution and the decline of the fishery resources,thus can’t meet the demands of people. The researchers have successively separated multiple genes that involved in LCPUFA synthesis and clarified the multiple LCPUFA synthetic pathways. LCPUFAs,especially DHA and EPA,have been successfully produced in monocot and dicot plants through genetic engineering. We summarized the latest research progress of LCPUFAs bio-synthesis and genetic engineering in higher plants,and analyzed the issues of LCPUFA synthesis,then proposed the possible solutions,and had an prospect of LCPUFA by genetic engineering.

Key words: long-chain polyunsaturated fatty acids, EPA, DHA, transgenic plant

LI Wen-zong,WANG Lei. Research Progress on Genetic Engineering for Long-chain Polyunsaturated Fatty Acids EPA and DHA[J]. Biotechnology Bulletin, 2016, 32(8): 1-7.

References

[1] Schmitz G, Ecker J. The opposing effects of n-3 and n-6 fatty acids. [J]. Prog Lipid Res, 2008, 47（2）：147-155.
[2] 张洪涛, 单雷, 毕玉平. n-6和n-3多不饱和脂肪酸在人和动物体内的功能关系[J]. 山东农业科学, 2006（2）：115-1201.
[3] 陈银基, 周光宏, 徐幸莲. N-3 多不饱和脂肪酸对疾病的预防与治疗作用[J]. 中国油脂, 2006（9）：31-34.
[4] Saravanan P, Davidson NC. Cardiovascular effects of marine omega-3 fatty acids[J]. Lancet, 2010, 376（9740）：540-550.
[5] Das UN, Fams MD. Long-chain polyunsaturated fatty acids in the growth and development of the brain and memory[J]. Nutrition, 2003, 19（1）：62-65.
[6] Crawford MA, et al. Are deficits of arachidonic and docosahexaenoic acids responsible for the neural and vascular complications ofpreterm babies?[J]. Am J Clin Nutr, 1997, 4 Suppl：1032S-1041S.
[7] Spite M. Deciphering the role of n-3 polyunsaturated fatty acid-derived lipid mediators in health and disease[J]. Proc Nutr Soc, 2013, 72（4）：441-450.
[8] 曾硕士, 江黎明, 元冬娟. 脂肪酸去饱和酶的研究进展[J]. 生命科学, 2008, 20（5）：816-820.
[9] Williams CM, Burdge G. Long-chain n-3 PUFA：plant v. marine sources[J]. Proc Nutr Soc, 2006, 65（1）：42-50.
[10] Cressey D. Aquaculture：Future fish[J]. Nature, 2009, 458 （7237）：398-400.
[11] 郝颖, 汪之和. EPA、DHA的营养功能及其产品安全性分析[J]. 现代食品科技, 2006, 22（3）：180-182.
[12] Brustad M, Sandanger TM, Nieboer E. 10th anniversary review：when healthy food becomes polluted-implications for public healthy and dietary advice[J]. J Environ Monit, 2008, 10：422-427.
[13] Jacobs MN, Santillo D, Johnston PA, et al. Organochlorine residues in fish oil dietary supplements：comparison with industrial grade oils[J]. Chemosphere, 1998, 37：1709-1721.
[14] Bourdon JA, Bazin TM, Arnason TT, et al. Polychlorinated biphenyls（PCBs）contamination and aryl hydrocarbon receptor（AhR）agonist activity of omega-3 polyunsaturated fatty acid supplements：implications for daily intake of dioxins and PCBs[J]. Food Chem Toxicol, 2010, 48：3093-3097.
[15] Tocher D. Issues surrounding fish as a source of ω-3 long chain polyunsaturated fatty acids[J]. Lipid Technol, 2009, 21：13-16.
[16] Venegas-Calerón M, et al. An alternative to fish oils： Metabolic engineering of oil-seed crops to produce omega-3 long chain polyu-nsaturated fatty acids[J]. Prog Lipid Res, 2010, 2：108-119.
[17] Qiu X, et al. Identification of a Delta 4 fatty acid desaturase from Thraustochytrium sp. involved in the biosynthesis of docosahexanoic acid by heterologous expression in Saccharomyces cerevisiae and Brassica juncea[J]. J Biol Chem, 2001, 34：31561-31566.
[18] Spyehalla JP, Kinney AJ, Browse J. Identification of an animal omega-3 fatty acid desatarase by heterologous expression in Arabidopsis[J]. Proc Natl Acad Sci USA, l997, 18：1 242-l 147.
[19] Damude HG, et al. Identification of bifunetional △ 12/x3 fatty acid desaturases for improving the ratio of x3 to x6 fatty acids in microbes and plants[J]. PNAS, 2006, 203：9 446-9 452.
[20] Sayanova OV, Napier JA. Eicosapentaenoic acid：biosynthetic routes and the potential for synthesis in transgenic plants[J]. Phytochemistry, 2004, 65（2）：147-158.
[21] Bentley R, Bennett JW. Constructing polyketides：from collie to combinatorial biochemistry[J]. Annu Rev Microbiol, 1999, 53：411-456.
[22] Abbadi A, et al. Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds：constraints on their accumulation[J]. Plant Cell, 2004, 16：2734-2748.
[23] Robert SS, Singh SP, Zhou XR, et al. Metabolic engineering of Arabidopsis to produce nutritionally important DHA in seed off[J]. Funct Plant Biol, 2005, 32：473-479.
[24] Wu GH, Truksa M, Datla N, et al. Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants[J]. Nat Bioteehnol, 2005, 23（8）：1013-1017.
[25] Kinney AJ, et al. Production of very long chain polyunsaturated fatty acids in oilseed plants：US, 2004/0172682 AI[P]. 2004-09-02.
[26] Parker-Barnes JM, et al. Identification an d characterization of an enzyme involved in the elonga- tion of n-6 and n-3 polyrunsaturated fatty acids[J]. PNAS, 2000, 97：8284-8289.
[27] Cheng B, et al. Towards the production of high levels of eicosapent-aenoic acid in transgenic plants：the effects of different host species, genes and promoters[J]. Transgenic Res, 2010, 2：221-229.
[28] Wahlroos T, Susi P, Solovyev A, et al. Increase of histidine content in Brassica rapa subsp. oleifera by over-expression of histidine-rich fusion proteins[J]. Mol Breed, 2004, 14：455-462.
[29] Meesapyodsuk D, Reed DW, et al. Primary structure, regioselecti-vity, and evolution of the membrane-bound fatty acid desaturases of Claviceps purpurea[J]. J Biol Chem, 2007, 28：20191-20199.
[30] Bauer J, Wu GH, Qiu X. Isolation and characterization of a novel pythium omega-3 desaturase with specificity to all omega-6 fatty acids longer than 18 carbon chains：US, 2015/0211032 AI[P]. 2015-17-30.
[31] Ruiz-Lopez N, Haslam RP, et al. Successful high-level accumulat-ion of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop[J]. Plant J, 2014, 2：198-208.
[32] Petrie JR, et al. Metabolic engineering plant seeds with fish oil-like levels of DHA[J]. PLoS One, 2012, 7（11）：| 49165.
[33] Qi B, Fraser T, Mugford S, et al. Production of very long chain polyunsaturated omega -3 and omega-6 fatty acids in plants[J]. Nat Bioteehnol, 2004, 22：739-745.
[34] 戴晓峰, 肖玲, 武玉花, 等, 植物脂肪酸去饱和酶及其编码基因研究进展[J]. 植物学通报, 2007（1）：105-113.
[35] Domergue F, et al. Relief for fish stocks：oceanic fatty acids in transgenic oilseeds[J]. Trends Plant Sci, 2005, 10：112-116.
[36] Sayanova O, et al. The role of Δ6-desaturase acyl-carrier specificity in the efficient synthesis of long-chain polyunsaturated fatty acids in transgenic plants[J]. Plant Biotechnol J, 2012, 2：195-206.
[37] Jaworski J, Cahoon EB. Industrial oils from transgenic plants[J]. Curr Opin Plant Biol, 2003, 6（2）：178-84.
[38] Gómez Candela C, Bermejo López LM, et al. Importance of a bala- nced omega 6/omega 3 ratio for the maintenance of health：nutri-tional recommendations. [J]. Nutr Hosp, 2011, 2：323-329.
[39] 刘汉梅, 何瑞, 张怀渝, 等. 玉米同义密码子偏爱性分析[J]. 农业生物技术学报, 2 01 0, 1 8（3）：456-461.
[40] 陈殊贤, 郑晓辉. 微藻油和鱼油中DHA的特性及应用研究进展[J]. 食品科学, 2013, 34（21）：439-444.