Current Progress on Ribosome Display

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.004

Abstract

Abstract: Ribosome display is a cell-free system for the selection of proteins and peptides from large libraries. It uses the principle of coupling the translated proteins（phenotypes）and their corresponding mRNA（genotypes）to ribosome and the stable protein-ribosome-mRNA complexes are formed. Then,the functional proteins and the corresponding encoding mRNAs are obtained by the simultaneous affinity separation of ligands,and they are converted and amplified as DNA for further expressions of related proteins,as well as selection of antibody and protein libraries,in vitro modification of proteins. It may display very large libraries while it is not limited by bacterial transformation. It is also suitable for screening the toxic,proteolytically sensitive,and unstable proteins,and allows the incorporation of modifying amino acids at defined loci. In this paper,research progress on ribosome display systems and their applications in the selection and evolution of proteins are reviewed.

Key words: ribosome display, antibody, selection, protein evolution

GUO Yuan. Current Progress on Ribosome Display[J]. Biotechnology Bulletin, 2016, 32(8): 22-27.

References

[1] Smith GP. Filamentous fusion phage：novel expression vectors that display cloned antigens on the virion surface[J]. Science, 1985, 228（4705）：1315-1317.
[2] Santich BH, Liu H, Liu C, et al. Generation of TCR-like antibodies using phage display[J]. Methods Mol Biol, 2015, 1348：191-204.
[3] Shusta, EV, VanAntwerp, J, Wittrup KD. Biosynthetic polypeptide libraries[J]. Curr Opin Biotechnol, 1999, 10（2）：117-122.
[4] Van Deventer JA, Yuet KP, Yoo TH, et al. Cell surface display yields evolvable, clickable antibody fragments[J]. Chembiochem, 2014, 15（12）：1777-1781.
[5] Soga K, Abo H, Qin SY, et al. Mammalian cell surface display as a novel method for developing engineered lectins with novel characteristics[J]. Biomolecules, 2015, 5（3）：1540-1562.
[6] Hanes J, Pluckthun A. In vitro selection and evolution of functional proteins by using ribosome display[J]. Proc Natl Acad Sci, USA, 1997, 94（10）：4937-4942.
[7] He M, Taussig MJ. ARM complexes as efficient selection particles for in vitro display and evolution of antibody combining sites[J]. Nucleic Acids Res, 1997, 25：5132-5134.
[8] Chen L, Kutskova YA, Hong F, et al. Preferential germline usage and VH/VL pairing observed in human antibodies selected by mRNA display[J]. Protein Eng Des Sel, 2015, 28（10）：427-435.
[9] Tokunaga M, Shiheido H, Hayakawa I, et al. Hereditary spastic paraplegia protein spartin is an FK506-binding protein identified by mRNA display[J]. Chem Biol, 2013, 20（7）：935-942.
[10] Mattheakis LC, Bhatt RR, Dower WJ. An in vitro polysome display system for identifying ligands from very large peptide libraries[J]. Proc Natl Acad Sci USA, 1994, 91（19）：9022-9026.
[11] Taussig MJ, Groves MAT, Menges M, et al. ARM complexes for in vitro display and evolution of antibody combining sites[M]//Monoclonal Antibodies：A Practical Approach. In Shepherd P, Dean C Eds. Oxford：Oxford University Press, 2000：91-109.
[12] Irving RA, Coia G, Roberts A, et al. Ribosome display and affinity maturation：from antibodies to single V-domains and steps towards cancer therapeutics[J]. J Immunol Methods, 2001, 248：31-45.
[13] He M, Taussig MJ. Ribosome display：Cell-free protein display technology[J]. Brief Funct Genomic Proteomic, 2002, 1（2）：204-212.
[14] Ellman J, Mendel D, Anthony-Cahill S, et al. Biosynthetic method for introducing unnatural amino acids site-specifically into proteins[J]. Methods Enzymol, 1991, 202：301-336.
[15] Kanamori T, Fujino Y, Ueda T. PURE ribosome display and its application in antibody technology[J]. Biochimica et Biophysica Acta, 2014, 1844：1925-1932.
[16] Griffiths AD, Tawfik DS. Man-made enzymes—from design to in vitro compartmentalization[J]. Cur Opin Biotech, 2000, 11（4）：338-353.
[17] Levy M, Ellington AD. Directed evolution of streptavidin variants using in vitro compartmentalization[J]. Chemi Biol, 2008, 15（9）：979-989.
[18] Tay Y, Ho C, Droge P, et al. Selection of bacteriophage lambda integrases with altered recombination specificity by in vitro compartmentalization[J]. Nucleic Acids Res, 2010, 38（4）：e25.
[19] Ogawa A, Hayami M, Sando S, et al. A concept for selection of codon-suppressor tRNAs based on read-through ribosome display in an in vitro compartmentalized cell-free translation system[J]. J Nucleic Acids, 2012, 2012：538129.
[20] Chin SE, Ferraro F, Groves M, et al. Isolation of high-affinity, neutralizing anti-idiotype antibodies by phage and ribosome display for application in immunogenicity and pharmacokinetic analyses[J]. J Immunol Methods, 2015, 416：49-58.
[21] Zhao L, Ning BA, Bai JL, et al. Selection of bisphenol A-single-chain antibodies from a non-immunized mouse library by ribosome display[J]. Analytical Biochemistry, 2015, 488：59-64.
[22] Luo YH, Xia YX. Selection of single-chain variable fragment antibodies against fenitrothion by ribosome display[J]. Anal Biochem, 2012, 421：130-137.
[23] Chen F, Zhao Y, Liu M, et al. Functional selection of hepatitis C virus envelope E2-binding peptide ligands by using ribosome display[J]. Antimicrob Agents Chemother, 2010, 54（8）：3355-3364.
[24] Zhao XL, Chen WQ, Yang ZH, et al. Selection and affinity maturation of human antibodies against rabies virus from a scFv gene library using ribosome display[J]. J Biotech, 2009, 144：253-258.
[25] Zhou L, Mao WP, Fen JA, et al. Selection of scFvs specific for the HepG2 cell line using ribosome display[J]. J Biosci, 2009, 34 （2）：221-226.
[26] Sun YN, Ning BA, Liu M, et al. Selection of diethylstilbestrol-specific single-chain antibodies from a non-immunized mouse ribosome display library[J]. PLoS One, 2012, 7（3）：e33186.
[27] Qi YH, Wu CM, Zhang SX, et al. Selection of anti-sulfadimidine specific ScFvs from a hybridoma cell by eukaryotic ribosome display[J]. PLoS One, 2009, 4（7）：e6427.
[28] Cheng HW, Chen YF, Yang Y, et al. Characterization of anti-citrinin specific ScFvs selected from non-immunized mouse splenocytes by eukaryotic ribosome display[J]. PLoS One, 2015, 10（7）：e0131482.
[29] Skirgaila R, Pudzaitis V, Paliksa S, et al. Compartmentalization of destabilized enzyme-mRNA-ribosome complexes generated by ribosome display：a novel tool for the directed evolution of enzymes[J]. Protein Eng Des Sel, 2013, 26（7）：453-461.
[30] Whaley SR, English DS, Hu EL, et al. Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly[J]. Nature, 2000, 405：665-668.
[31] Ejima H, Matsumiya K, Sawada T, et al. Conjugated polymer nanoparticles hybridized with the peptide aptamer[J]. Chem Commun, 2011, 47：7707-7709.
[32] Li Z, Uzawa T, Zhao H, et al. In vitro selection of peptide aptamers using a ribosome display for a conducting polymer[J]. J Biosci Bioeng, 2014, 117（4）：501-503.
[33] Li Z, Uzawa T, Tanaka T, et al. In vitro selection of peptide aptamers with affinity to single-wall carbon nanotubes using a ribosome dis-play[J]. Biotechnol Lett, 2013, 35：39-45.