The Current Status and Future Perspectives of Production of Biopharmaceuticals in Escherichia coli

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.007

Abstract

Abstract: Escherichia coli is the most preferred microorganism to express heterologous protein for therapeutic use,as around 30% of the approved therapeutic proteins are currently being produced using it as a host. Due to its rapid growth,high yield,cost-effectiveness,and easy scale-up process,E. coli is chosen as an expression host in biotech industry for large-scale production of proteins. However,codon preference in E. coli and absences of post-translational modifications,such as glycosylation,phosphorylation and proteolytic processing limit its use for the production of slightly complex recombinant biopharmaceuticals. On purpose of helping E. coli to produce more complex and also glycosylated proteins for therapeutic use,this review summarizes the several novel technological advancements meeting the requirements of biotech industry,mainly focusing on the process of E. coli glycosylating heterologous proteins and expressing complex proteins including full-length glycosylated antibodies via those advancements. Further potential problems and prospects of futures researches are also discussed.

Key words: Escherichia coli, biopharmaceuticals, codon preference, molecular chaperones

CAI Dong-mei, GONG Guo-li. The Current Status and Future Perspectives of Production of Biopharmaceuticals in Escherichia coli[J]. Biotechnology Bulletin, 2016, 32(8): 34-40.

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