Sequence Divergence and Analysis of Expression Difference of Vacuolar Invertase Gene EhvINV from Different Resistant Populations in Elsholtzia haichowensis

doi:10.13560/j.cnki.biotech.bull.1985.2016.10.021

Abstract

Abstract: Specific primers were designed to clone the cDNA sequences according to the EhNvINV（JX500755）and EhCvINV（JX500756）from GenBank. The DNA and deduced amino acid sequences were analyzed by bioinformatics methods. The three-dimensional structures were constructed by homologous modeling. The structures of vacuolar invertase from two populations of E. haichowensis and non-tolerant population with each single point mutation in complex with sucrose were simulated by AutoDock 4.0. Transcript expression of EhNvINVand EhCvINV under copper tress was analyzed by real-time PCR. The results showed that there were two divergent amino acids at position 114 and 346 between EhNvINV and EhCcINV. The three-dimensional structures were exactly similar between EhNvINV and EhCvINV. It showed difference at Glu114/Gln114 and Leu346/Pro346,which were divergent sites. The structures of catalytic active center of EhNvINV,EhCvINV,and simulated mutants（EhNvINV-E114Q,EhNvINV-L346P）binding with sucrose showed no significant differences. However,there were differences on spatial position. The result of real-time PCR indicated that the transcript expression of EhCvINV induced by copper stress after 7 days,however,the transcript expression of EhNvINV inhibited by copper stress after 7 days.

Key words: Elsholtzia haichowensis, vacuolar invertase, copper stress, transcript expression

CAI Shen-wen, XU Zhong-rui, XIONG Zhi-ting, WANG Jia-zhen, CHEN Yao. Sequence Divergence and Analysis of Expression Difference of Vacuolar Invertase Gene EhvINV from Different Resistant Populations in Elsholtzia haichowensis[J]. Biotechnology Bulletin, 2016, 32(10): 170-179.

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