Effect of Cryoprotectant on the Survival Rate of Freeze-dried Pseudomonas sp. Powder

doi:10.13560/j.cnki.biotech.bull.1985.2017-0215

Abstract

Abstract: The cryoprotectant presents remarkable effect on increasing the survival rate and stability of freeze-dried Pseudomonas sp. powder. Here single-factor and orthogonal experimental designs were conducted to screen the optimal combination of cryoprotectant. The results showed that the survival rate reached 90% when the concentration of skim milk powder,sorbitol,glycerol,and L-sodium ascorbate were 10%,3%,1%,2%,and was 50.2% while stored for 6 months at 25℃.

Key words: Pseudomonas sp., cryoprotectant, survival rate

LI Li,ZHAO Peng-nian,ZHU Xi-kun. Effect of Cryoprotectant on the Survival Rate of Freeze-dried Pseudomonas sp. Powder[J]. Biotechnology Bulletin, 2017, 33(9): 231-234.

References

[1] Glick BR, Karaturovíc DM, Newell PC. A novel procedure for the rapid isolation of plant growth-promoting Pseudomonas[J] . Canadian Journal of Microbiology, 1995, 41（6）：533-536.
[2] Albano LJ, Macfie SM. Investigating the ability of Pseudomonas fluorescens UW4 to reduce cadmium stress in Lactuca sativa via an intervention in the ethylene biosynthetic pathway[J] . Canadian Journal of Microbiology, 2016, 62（12）：1057-1062.
[3] Nadeem SM, Zahir ZA, Naveed M, et al. Rhizobacteria containing ACC-deaminase confer salt tolerance in maize grown on salt-affected fields[J] . Can J Microbiol, 2009, 55（11）：1302-1309.
[4] Ahmad M, Zahir ZA, Asghar HN, et al. Inducing salt tolerance in mung bean through coinoculation with rhizobia and plant-growth-promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase[J] . Canadian Journal of Microbiology, 2011, 57（7）：578-589.
[5] Manikandan R, Raguchander T. Fusarium oxysporum f. sp. lycopersici retardation through induction of defensive response in tomato plants using a liquid formulation of Pseudomonas fluorescens（Pf1）[J] . Eur J Plant Pathol, 2014, 140（3）：469-480.
[6] Khan MR, Anwer MA, Shahid S. Management of gray mold of chickpea, Botrytis cinerea with bacterial and fungal biopesticides using different modes of inoculation and application[J] . Biological Control, 2011, 57（1）：13-23.
[7] 张书景, 李坚, 李依丽, 等. 恶臭假单胞菌生物滴滤塔净化甲苯废气的研究[J] . 环境科学, 2007, 28（8）：1866-1872.
[8] Mishra S, Singh SN. Microbial degradation of n-hexadecane in mineral salt medium as mediated by degradative enzymes[J] . Bioresource Technology, 2012, 111：148-154.
[9] Shi GY, Yin H, Ye JS, et al. Aerobic biotransformation of decabromodiphenyl ether（PBDE-209）by Pseudomonas aeruginosa[J] . Chemosphere, 2013, 93（8）：1487-1493.
[10] Srivastava AK, Singh T, et al. Induced resistance and control of charcoal rot in Cicer arietinum（chickpea）by Pseudomonas fluorescens[J] . Can J Bot, 2001, 79（7）：787-795.
[11] 朱希坤, 彭湃, 李小明, 等. 假单胞菌及其用途：中国, CN201410665101. 0[P] . 2015-02-18.
[12] Palmfeldt J, R?dstr?m P, Hahn-H?gerda B. Optimisation of initial cell concentration enhances freeze-drying tolerance of Pseudomonas chlororaphis[J] . Cryobiology, 2003, 47（1）：21-29.
[13] Costa E, Usall J, Teixido N, et al. Effect of protective agents, rehydration media and initial cell concentration on viability of Pantoea agglomerans strain CPA-2 subjected to freezedrying[J] . Journal of Applied Microbiology, 2006, 89（5）：793-800.
[14] Clement MT. Effects of freezing, freeze-drying, and storage in the freeze-dried and frozen state on Viability of Escherichia coli Cells[J] . Can J Microbiol, 1961, 7（1）：99-106.
[15] Stephan D, Silva APMD, Bisutti IL. Optimization of a freeze-drying process for the biocontrol agent Pseudomonas sp. and its influence on viability, storability and efficacy[J] . Biological Control, 2016, 94：74-81.
[16] Souzu H. Basic aspects and industrial strategies for the preservation of microorganisms by freezing and drying in “Freeze-drying/lyophilization of pharmaceutical and biological products”[M] . New York：Marcel Dekker, 1999, 22-35.
[17] Prakash O, Nimonkar Y, et al. Practice and prospects of microbial preservation[J] . FEMS Microbiol Lett, 2013, 339（1）：1-9.
[18] Hubálek Z. Protectants used in the crypreservation of microorganisms[J] . Cryobiology, 2003, 46（3）：205-229.
[19] 山丽杰, 田洪涛, 等. 浓缩型乳酸菌发酵剂制备中几个技术关键问题的探讨[J] . 中国乳品工业, 2002, 30（5）：66-69.