Isolation，Identification and Characterization of a PCBs-degrading Bacterium

doi:10.13560/j.cnki.biotech.bull.1985.2017-1038

Abstract

Abstract: This study is aimed to investigate the degradation characteristics of PCBs-degrading strain screened and isolated from estuary utilizing biphenyl as sole carbon and energy source. Pseudomonas sp. P-6-5 was investigated in more details of growth and substrate utilization pattern towards BPH and PCB3. The optimal growth conditions for strain P-6-5 were pH 7.0 and salinity 35 g/L. The use of BPH and PCB3 as an inducing agent promoted the growth of degrading bacteria. Strain P-6-5 had the transformation ability to PCB3 with substrate concentration of 10-100 mg/L. The degradation rate of PCB3 by strain P-6-5 was 95.3% in cultures containing 10 mg/L PCB3 and the maximum degradation speed was 1.9 mg/L·h. The PCBs substrate spectrum was assessed by mix13（a mixture of 13 PCB congeners）and the data showed that P-6-5 had the ability to degrade up to four chlorinated polychlorinated biphenyls. Combined with product analysis，we speculated that strain P-6-5 may have the ability to mineralize PCB3. In conclusion，Pseudomonas sp. P-6-5 has the characteristics of seawater bacteria and shows a broad range of substrate spectrum. These results postulate that the P-6-5 is an efficient PCBs-degrading strain，and has a great potential to be utilized in the bioremediation of PCBs pollution.

Key words: polychlorinated biphenyls, biodegradation, Pseudomonas sp., 4-chlorobenzoic acid

SUN Gui-ting ,CHEN Hong-yun ,ZHAO Ling-chao ,HU Xiao-ke ,CHEN Ying. Isolation，Identification and Characterization of a PCBs-degrading Bacterium[J]. Biotechnology Bulletin, 2018, 34(6): 141-148.

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