Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (10): 88-96.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0448

• Review • Previous Articles     Next Articles

Research Progresses on Strain Construction of Xylose Isomerase-based Recombinant Xylose-fermenting Saccharomyces cerevisiae

LI Yun-cheng1, MENG Fan-bing1, GOU Min2, SUN Zhao-yong2, TANG Yue-qin2   

  1. 1. College of Pharmacy and Bioengineering,Chengdu University,Chengdu 610106
    2. College of Architecture and Environment,Sichuan
    University,Chengdu 610207
  • Received:2017-05-30 Online:2017-10-29 Published:2017-10-29
  • Contact: 汤岳琴,女,博士,教授,研究方向:环境生物工程;E-mail:tangyq@scu.edu.cn

Abstract: It is of utmost significant to construct industrial xylose-fermenting Saccharomyces cerevisiae strains for lignocellulosic bioethanol production through the heterologous expression of xylose metabolic pathway. Xylose isomerase pathway is regarded as the most promising pathway expressing in S. cerevisiae,since there is no xylitol accumulation resulted from the coenzyme imbalance in xylose redox pathway. In heterologous expression of xylose isomerase,selecting an industrial S. cerevisiae strain as initial strain is of outstanding advantages for lignocellulosic bioethanol production. Concurrently,improving the expression efficiency of xylose isomerase gene(xylA)is vital for constructing a robust xylose fermentation strain. In addition,the deletion of GRE3,strengthening of xylose transport and rational modification of xylose metabolic pathway during the metabolic modification of strain XI effectively improved the xylose fermentation capacity of the recombinant strains. Besides,evolutionary engineering also increased the xylose fermentation efficiency of XI strains. Furthermore,the omics technologies have presented their power in explaining the mechanism and developing the modification strategies of xylose metabolism. This paper reviews the research progresses on the expressions of xylose isomerase pathway in S. cerevisiae and analyzes the issues in the relevant studies.

Key words: Saccharomyces cerevisiae, fuel ethanol, xylose isomerase, strain construction