Comparison on Microstructure and Chlorophyll Fluorescence Parameter in Cassava

doi:10.13560/j.cnki.biotech.bull.1985.2017-0673

Abstract

Abstract: In this study,wild cassava（Manihot glaziovii）,wild relative W14（M. esculenta ssp. flabellifolia）and SC205（M. esculenta cv. SC No.205）were used as materials. Leaf anatomical structures and chlorophyll fluorescence parameter were analyzed in order to show the difference in its structure and photosynthetic activity. The microstructures of cassava functional leaves were observed by paraffin sections,95% ethanol extraction method to extract chlorophyll directly,using PAM-2500 to measure chlorophyll fluorescence kinetics parameters of light systemII（PS II）. Result showed that wild cassava and SC205 leaves were with "unique" green bundle sheath cell structure,but no typical “kranz structure” of C4 plant. The overall shape of green bundle sheath cells in W14 was slightly like the “kranz structure”,but was not typical as C4 plant,while mesophyll cells were adjacent to the external of the green bundle sheath and they were arranged at slightly loose. Content of chlorophyll A and total chlorophyll in W14 leaves were significantly lower than wild cassava and SC205. Chlorophyll fluorescence parameter ΦPS II in them can be sequenced as SC205 > wild cassava> W14;however,Fv/Fm in W14 was higher than wild cassava and SC205,speculated that W14 might have a greater potential of photosynthetic capacity. Wild cassava and SC205 belong to C3 plant,W14 was a kind of cassava germplasm between C3 and C4. Their photosynthetic efficiencies in ΦPS II were in descending order of SC205,wild cassava,and W14.

Key words: Manihot esculenta Crantz, leaves, microstructure, chlorophyll fluorescence, photosynthetic efficiency

AN Fei-fei, CHEN Ting, YANG Long, CHEN Song-bi. Comparison on Microstructure and Chlorophyll Fluorescence Parameter in Cassava[J]. Biotechnology Bulletin, 2018, 34(1): 104-109.

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