Alleviation Effect of Exogenous Jasmonic Acid on Detrimental Effect of Rhododendron chrysanthum Pall Under UV-B Radiation

doi:10.13560/j.cnki.biotech.bull.1985.2019-0541

Abstract

Abstract: The objective of this work is to explore the feasible role of jasmonic acid in UV-B radiation and the mechanism of its mitigation. Jasmonic acid pretreated Rhododendron chrysanthum for 4 d,then PAR and UV-B radiation was conducted for 48 h,and chlorophyll fluorescence parameters,superoxide dismutase（SOD）,catalase（CAT）,malondialdehyde（MDA）,NADPH oxidase and anthocyanin were measured.UV-B radiation induced a significant reduction of Fm,Fv/Fm,Fv'/Fm',Fv/Fo,qP,anthocyanin content,and CAT activity,and increased the decreased amplitude of qP and rETR rapid photoresponse curves,while significantly increased the activity of SOD and NADPH oxidase. JA pretreatment obviously mitigated the detrimental effect of UV-B on R. chrysanthum by increasing Fm,Fv/Fm,Fv'/Fm',Fv/Fo,qP,anthocyanin content,and SOD and NADPH oxidase activities,and maintained MDA content and CAT activity at the control level,and the qP,NPQ and rETR fast light response curves presented the optimal state. In conclusion,these results confirm that the hypothesis of the external application of jasmonic acid can make R. chrysanthum resistant to stress,partially offset the negative effect of UV-B radiation on R. chrysanthum.

Key words: Rhododendron chrysanthum Pall., UV-B radiation, jasmonic acid, chlorophyll fluorescence, antioxidant enzyme.

LIANG Dong-yu, WANG Yu-jia, SU Ao-chun, XU Hong-wei, ZHOU Xiao-fu. Alleviation Effect of Exogenous Jasmonic Acid on Detrimental Effect of Rhododendron chrysanthum Pall Under UV-B Radiation[J]. Biotechnology Bulletin, 2019, 35(10): 64-70.

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