Cloning of a HD-ZIP Transcription Factor Gene HD20 and A Preliminary Study on Function of Fiber Differentiation in Tobacco

doi:10.13560/j.cnki.biotech.bull.1985.2017-0928

Abstract

Abstract: To understand the role of HD20 in the specification of fiber differentiation，we cloned the HD20 gene from tobacco by RT-PCR method. The cDNA sequence was cloned according to specific primers designed by the genomic database in tobacco and named asHD20（GenBank accession number：KC339003）. Sequence analysis showed that the cDNA sequence of HD20 was 762 bp and encoded 253 amino acids. Subcellular localization assays showed that it appeared in the nuclei. The HD-ZIP protein contained two significant domains，including the HD domain with 60 amino acids at the N-terminus and a ZIP matrix with 28 amino acids. We introduced HD20 into the expression vector pSH-737，transferred it by Agrobacterium mediated-dip flower method，and obtained positive transgenic plants for paraffin sections. The results of paraffin section showed that the xylem of stem was more developed than the control group. In conclusion，the result provided evidence that HD20 plays a role in the spatial control of fiber differentiation. The analysis of the regulatory effect of HD20 on tobacco and Arabidopsis helps to create a theoretical base for studying HD20 function.

Key words: tobacco, HD20, paraffin section, fiber differentiation

GAO Jie ,TONG Shuo-qiu ,ZHONG Jie ,GONG Xian, WU Yong-jun. Cloning of a HD-ZIP Transcription Factor Gene HD20 and A Preliminary Study on Function of Fiber Differentiation in Tobacco[J]. Biotechnology Bulletin, 2018, 34(6): 84-89.

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