Construction and Biological Characterization of Gene dlp Deletion Mutant of Deinococcus radiodurans R1

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.023

Abstract

Abstract: Deinococcus radiodurans（DR）has drawn growing attentions for its superior resistance to abiotic stress. To investigate the effect of the hydrophilic protein Dlp in cell tolerance to abiotic stress,the dlp deletion mutant strain（Δdlp）was obtained by fusion PCR and homologous recombination in vivo. The assays of wild strain DR and mutant Δdlp were analyzed under abiotic stress. The results showed that the deletion of dlp gene in D. radiodurans led cells to be more sensitive to high-salt and oxidative stresses. The in vitro protective effect of Dlp protein on the activities of MDH and LDH was measured under oxidative stress condition;and the data demonstrated that the addition of Dlp protein alleviated the loss of activities of MDH and LDH under oxidative stress. Our findings indicated that hydrophilic protein Dlp enhanced the resistance of D. radiodurans to abiotic stresses,and functioned as chaperone-like protein to protect enzyme activity under abiotic stresses.

Key words: Deinococcus radiodurans, gene mutant, hydrophilic protein Dlp, abiotic stress, enzyme activity

LIU Xiao-li, JIANG Shi-jie, XUE Dong, LIU Ying-ying, WU Xiao-li, FENG Shuai, HAN Jia-hui, WANG Yu-zhou, PING Shu-zhen, WANG Jin. Construction and Biological Characterization of Gene dlp Deletion Mutant of Deinococcus radiodurans R1[J]. Biotechnology Bulletin, 2017, 33(2): 155-163.

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