Effect of Ferritin DrfE on Antioxidant Enzyme Activity in Deinococcus radiodurans

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.024

Abstract

Abstract: To investigate the function of the ferritin DrfE encoded by gene drfE in Deinococcus radiodurans,we constructed the complemented strain pdrfE∷Δ,and compared the stress resistances of the wild-type WT,mutant ΔdrfE and complemented strain pdrfE∷Δ under different doses of hydrogen peroxide（H₂O₂）and NaCl. Also,the Fe²⁺concentration and antioxidant enzyme activities of three strains were measured under oxidative stress. The results showed that the deletion of gene drfE led the strain to be more sensitive to oxidative and high-salt stresses and the complementation of gene drfE restored the resistance of the strain to oxidative and salt stress. Furthermore,the deletion of drfEresulted in the Fe²⁺ concentration in D. radiodurans cell increased from 232 μmol/L to 293 μmol/L in vivo. After treated with 80 mmol/L H₂O₂for 30 min,the CAT and SOD activities of the mutant ΔdrfE decreased by 32.74% and 41.3%,respectively,than that of wild-type. The above findings implied that the ferritin DrfE might be involved in iron metabolic pathway of D. radiodurans and played a key role in antioxidant system.

Key words: Deinococcus radiodurans, ferritin DrfE, abiotic stress, antioxidant enzyme, iron ion

WU Xiao-li, LIU Ying-ying, JIANG Shi-jie, CHEN Yun, LIU Xiao-li, WANG Yu-zhou, PING Shu-zhen, WANG Jin. Effect of Ferritin DrfE on Antioxidant Enzyme Activity in Deinococcus radiodurans[J]. Biotechnology Bulletin, 2017, 33(2): 164-171.

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