Current Issues and Progress in the Application of CRISPR/Cas9 Technique

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.009

Abstract

Abstract: CRISPR/Cas9[clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9]is a RNA-guided genome-editing technique,which can be applied in the deletion,insertion or replacement of specific DNA sequence thus to verify the function of genes or remedy pathogenic genes. Owing to its simplicity,affordability,multiple-site editing and scalability,the technique of CRISPR/Cas9 is undergoing rapid development and perfection and has been one of the first three prevailing genome-editing technology following that of Zinc finger nucleases and transcription activator-like effector nucleases. In this review,we focus on the current issues of CRISPR/Cas9,including low efficiency of precise-editing,sequence-recognizing limitation from PAM,off-target,unavoidable mosaicism,together with corresponding tactics for improvement. Our aim is to provide the objective understanding on the current disadvantage of CRISPR/Cas9 technique to researchers and the systematic review for reasonable application and improvement of this technology.

Key words: CRISPR/Cas9, genome-editing, precise-editing, off-target, mosaicism

YUAN Wei-xi, YU Yun-mei, HU Chun-cai, ZHAO Zu-guo. Current Issues and Progress in the Application of CRISPR/Cas9 Technique[J]. Biotechnology Bulletin, 2017, 33(4): 70-77.

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