Preliminary Study on the Function of Polyphenol Oxidase in Cassava Resistance to Mite

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.018

Abstract

Abstract: So far,the function of polyphenol oxidase（PPO）in cassava resistance to mite has not been understood clearly. In order to validate this function,we used quantitative real-time PCR（qPCR）and enzymatic assay to determine the changes of expression and activities of PPO in either cassava or Tetranychus cinnabarinus after the mite-resistant and mite-susceptible cassava cultivars were damaged by T. cinnabarinus. The results showed that,in one hand,compared to those in the same leaves before damaged,the transcriptions of MePPO and total activities of PPO only increased to 0.99-,1.02-fold and 1.05-,1.03-fold while the mite-susceptible cassava cultivar BRA900 was damaged by T. cinnabarinus for 1 d and 8 d,respectively. However,the corresponding values in 1 d- and 8 d-damaged leaves of mite-resistant cassava C1115 increased to 1.78-,1.74-fold and 1.74-,1.72-fold,respectively,thus significantly higher than those of mite-susceptible cassava. In the other hand,compared with those before feeding,the expressions of TcPPOand activities of PPO in T. cinnabarinus were 0.98-,0.97-fold and 0.98-,1.10-fold,respectively after feeding on BRA900 for 1 d and 8 d;while the corresponding values in T. cinnabarinus feeding on C1115 decreased to 0.52-,0.64-fold and 0.54-,0.57-fold,respectively. This study preliminarily validated the function of PPO in cassava resistance to T. cinnabarinus.

Key words: polyphenol oxidase, Tetranychus cinnabarinus, cassava, resistance to mite

LIANG Xiao, LU Fu-ping, LU Hui, WU Chun-ling, CAO Xian-hong, CHEN Qing. Preliminary Study on the Function of Polyphenol Oxidase in Cassava Resistance to Mite[J]. Biotechnology Bulletin, 2017, 33(4): 143-148.

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