Advances in Gene Engineering Technologies for Aspergillus oryzae

doi:10.13560/j.cnki.biotech.bull.1985.2018-0252

Abstract

Abstract: The filamentous fungus Aspergillus oryzae is an important strain in fermentation industry. It has a strong capacity of secreting protein and recognized food safety,therefore is widely used as a cell factory for the expression of foreign proteins. However,the production and secretion of foreign proteins by A.oryzae are limited by a number of bottlenecks,including transcription,translation,protein folding,translocation,degradation,transport,and secretion,etc. The existence of these problems makes it difficult to achieve the desired target in the production of foreign proteins by A.oryzae. In recent years,with the decipherment of the whole genome sequence,the basic research and the genetic engineering technologies related to the production of A. oryzae have been well developed,for example,improvement of the homologous recombination efficiency,application of selectable marker genes,large chromosome deletion technology,hyphal fusion technique,DNA microarray,etc. The development and establishment of these technologies provided a great deal of technical support for the industrial production and application of A. oryzae. Regarding the bottlenecks in the heterologous protein expression of A. oryzae,this article mainly elaborated several aspects such as the optimization of genetic transformation system and the increase of transformation efficiency,the construction of protease gene-deleted strains,the fusion expression strategy,and the optimization of its exogenous expression system to improve the production of foreign proteins. These advances in basic research and genetic engineering technology have greatly improved the production and application of A.oryzae,and also provide more effective ways and research space for the breeding of A.oryzae production strains in the future.

Key words: Aspergillus oryzae, cell factory, productionof foreign protein, genetic engineering technology

ZHANG Zhi-min, ZHUANG Miao, JIN Feng-jie. Advances in Gene Engineering Technologies for Aspergillus oryzae[J]. Biotechnology Bulletin, 2018, 34(9): 170-176.

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