Tolerance Elements and Their Application Progress on the Construction of Highly-efficient Microbial Cell Factory

doi:10.13560/j.cnki.biotech.bull.1985.2020-0259

Abstract

Abstract: Microorganisms,acting as cell factory,often encounter various stresses during the application process,which severely restrict cell viability and production performance. It is widely accepted that cell stress tolerance is a complex phenotype controlled by multiple intracellular metabolic pathways and diverse physiological systems. Therefore,the exploration and application for tolerance elements that endow strains with higher stress tolerance are an effective approach to construct highly-efficient microbial cell factory. At present,the reported tolerance elements mainly include the related genes that regulate cell wall and cell membrane,DNA repair,oxidative responses to stress,compatible solutes,energy generation and signal transduction as well as efflux pumps,heat shock proteins and global transcriptional factors. In this paper,tolerance elements and their application cases on the construction of highly-efficient microbial cell factory are reviewed. Meanwhile,the opportunities and challenges in practice are discussed.

Key words: tolerance element, stress condition, cell tolerance, cell factory, synthetic biology

CHANG Han-wen, ZHENG Xin-ling, LUO Jian-mei, WANG Min, SHEN Yan-bing. Tolerance Elements and Their Application Progress on the Construction of Highly-efficient Microbial Cell Factory[J]. Biotechnology Bulletin, 2020, 36(6): 13-34.

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