Dynamic Control：An Efficient Strategy for Metabolically Engineering Microbial Cell Factories

doi:10.13560/j.cnki.biotech.bull.1985.2020-0317

Abstract

Abstract: Dynamic control,consisting of 3 parts of input signal generator,biosensor and actuator,is one of the most efficient strategies for metabolic engineering optimization. The input signals can be metabolites and environmental changes,such as chemicals,RNA,temperature,light,etc. Biosensors are input signal-responsive genetic parts that transfer signals into specific ones that actuate the expression of target genes or can be used as input of downstream cascaded sensor systems. Here,we briefly introduce the basic principles and categories of dynamic control systems,and review the cases of microbial cell factory engineering by employing dynamic control,mainly including the advantages,recent progresses,construction and characterization. Meanwhile,we also discuss the potential opportunities and challenges of employing dynamic control in metabolically engineering microbial cell factories.

Key words: dynamic control, static control, metabolic engineering, biosensor, synthetic biology, microbial cell factory

YE Jian-wen, CHEN Jiang-nan, ZHANG Xu, Wu Fu-qing, CHEN Guo-qiang. Dynamic Control：An Efficient Strategy for Metabolically Engineering Microbial Cell Factories[J]. Biotechnology Bulletin, 2020, 36(6): 1-12.

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