Expression and Stress Tolerance Analysis of HbMC2 Gene from Hevea brasliensis in Yeast

doi:10.13560/j.cnki.biotech.bull.1985.2018-0298

Abstract

Abstract: Metacaspase-mediated programmed cell death plays an essential role in plant growth and development as well as resistances to biotic and abiotic stresses. Our previous study revealed that the rubber tree（Hevea brasliensis）metacaspase gene HbMC2 was regulated by abiotic stresses,such as drought,high salinity,etc. To investigate the function of HbMC2,the coding sequence of HbMC2 was amplified from rubber tree by RT-PCR and inserted into the pYES2 vector,and its yeast expression vector pYES2-HbMC2 was constructed. The plasmid of pYES2-HbMC2 was transformed into the yeast strain INVSc1 and the recombinant yeast INVSc1（pYES2-HbMC2）was obtained. PCR and RT-PCR analysis showed that HbMC2 gene was successfully introduced and expressed in the recombinant yeast when induced by galactose. Compared with the control yeast INVSc1（pYES2）,the recombinant yeast INVSc1（pYES2-HbMC2）demonstrated growth inhibition and its cell death rate increased under oxidative,drought,and salt stresses. These results indicate that expression of HbMC2 gene in yeast reduces tolerance to abiotic stress,i.e.,HbMC2 is a negative regulator of abiotic stress tolerance.

Key words: rubber tree, metacaspase, abiotic stress, yeast expression system, programmed cell death

LIU Hui, DENG Zhi, YANG Hong, DAI Long-jun, LI De-jun. Expression and Stress Tolerance Analysis of HbMC2 Gene from Hevea brasliensis in Yeast[J]. Biotechnology Bulletin, 2018, 34(9): 202-208.

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