Isolation,Identification of Carbon-fixing Bacteria and Determination of Their Carbon-fixing Abilities

doi:10.13560/j.cnki.biotech.bull.1985.2018-0544

Abstract

Abstract: Currently,as a biological carbon sequestration strategy,microbial recovery and fixing of CO₂ gas,has become the focus of interest in dealing with the major environmental problem of greenhouse effect. The purpose of this study is to separate and screen efficient carbon-fixing microbial strains. By using the carbon-free source inorganic medium,24 kinds of strains with CO₂ as carbon source were separated from facilities soil,activated sludge and biogas slurry. Then,the 8 strains of bacteria that grew faster were selected to conduct their morphological observations,physiological and biochemical responses as well as to perform cbbL gene and 16S rDNA sequencing analysis. Further,the sequencing results were compared with the data in BlAST database and the molecular characterization of the 8 carbon-fixing strains was carried out along with the detection of the strain content and the RubisCO enzyme activity. Finally,the C2-8R with the highest RubisCO activity among the 8 strains was selected for soil application test,and the carbon sequestration capacity of the C2-8R was determined through the detection of the RubisCO enzyme activity in soil. The above results revealed that carbon sequestration strains can be screened through carbon-free medium. The screened carbon-fixing bacteria are subordinate to the strains of Pseudomonas sp. and Methanotrophs sp.,and can be used to indicate the microbial carbon-fixing ability through RubisCO enzyme activity.

Key words: carbon-fixing microbial strains, cbbL gene, 16S rDNA, RubisCO activity

GUO Jun, FAN Fang-fang, WANG Li-ge, WU Ai-Lian, ZHENG Jun. Isolation,Identification of Carbon-fixing Bacteria and Determination of Their Carbon-fixing Abilities[J]. Biotechnology Bulletin, 2019, 35(1): 90-97.

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