The Diversity and Distribution Pattern of Bacterial Community in the Water of Yellow River Estuary

doi:10.13560/j.cnki.biotech.bull.1985.2017-0568

Abstract

Abstract: The Yellow River estuary，located in the confluence of Yellow River，Bohai Bay and Laizhou Bay，has unique geography and abundant microbial resources. However，the researches on bacterial community in this area were very limited. Here we used 16S rDNA clone libraries to explore the diversity and the characteristics of spatial distribution pattern of bacterial community in the unique estuarine ecosystem. The results demonstrated that different 16S rDNA clone libraries were constructed for 4 different water samples，and totally，we detected the bacteria in Yellow River estuary in 11 phyla，18 classes，39 families and 53 genera. The dominant bacteria were attributed in Proteobacteria （α-，β-，and γ-proteobacteria），Actinobacteria，Bacteroidetes and Cyanobacteria. Functional annotation results showed that aquatic bacteria played key roles in the cycling of carbon，nitrogen and sulfur elements. Species- or function-based cluster analyses indicated that samples could be divided into two different branches，A，B and C，D，respectively. Spearman correlation analysis and redundancy analysis （RDA） demonstrated that environmental factors （dissolved oxygen，salinity，and nitrogen nutrients） had significant effects on the structures and functions of aquatic bacterial community. The study shows that both the Yellow River and environmental factors drive the structure of bacterial community into varied spatial distribution patterns along the estuary. This study is a preliminary glimpse of bacterial diversity in the Yellow River estuary and adjacent seawater. It also provides data supports for further improvement in the rivers and marine environment of this area，and is beneficial to the microbial resource development and ecological protection of this water area.

Key words: Yellow River estuary, bacterial diversity, community function, 16S rDNA, clone library

WEI Guang-shan, ZHANG Jia-wei, LI Ming-cong, GAO Zheng. The Diversity and Distribution Pattern of Bacterial Community in the Water of Yellow River Estuary[J]. Biotechnology Bulletin, 2017, 33(10): 199-208.

References

[1]Mou X, Sun S, Edwards RA, et al. Bacterial carbon processing by generalist species in the coastal ocean[J]. Nature, 2008, 451(7179)：708-711.
[2] Glaubitz S, Lueders T, Abraham WR, et al. 13C-isotope analyses reveal that chemolithoautotrophic Gamma-and Epsilonproteobacteria feed a microbial food web in a pelagic redoxcline of the central Baltic Sea[J]. Environ Microbiol, 2009, 11(2)：326-337.
[3]Hargrave BT, Holmer M, Newcombe CP. Towards a classification of organic enrichment in marine sediments based on biogeochemical indicators[J]. Marine Pollution Bulletin, 2008, 56(5)：810-824.
[4]Anderson IC, Cairney J WG. Diversity and ecology of soil fungal communities：increased understanding through the application of molecular techniques[J]. Environ Microbiol, 2004, 6(8)：769-779.
[5]Schleifer KH. Microbial diversity：facts, problems and prospects[J]. Systematic and Mpplied Microbiology, 2004, 27(1)：3-9.
[6]姬洪飞, 王颖. 分子生物学方法在环境微生物生态学中的应用研究进展[J]. 生态学报, 2016, 36(24)：8234-8243.
[7]Xia N, Xia X, Liu T, et al. Characteristics of bacterial community in the water and surface sediment of the Yellow River, China, the largest turbid river in the world[J]. Journal of Soils and Sediments, 2014, 14(11)：1894.
[8]Li J, Wei G, Wang N, et al. Diversity and distribution of nirK-harboring denitrifying bacteria in the water column in the Yellow River estuary[J]. Microbes and Environments, 2014, 29(1)：107-110.
[9]Yan P, Li M, Wei G, et al. Molecular fingerprint and dominant environmental factors of nitrite-dependent anaerobic methane-oxidizing bacteria in sediments from the Yellow River Estuary, China[J]. PLoS One, 2015, 10(9)：e0137996.
[10]Wei G, Li M, Li F, et al. Distinct distribution patterns of prokaryotes between sediment and water in the Yellow River estuary[J]. Applied Microbiology and Biotechnology, 2016, 100(22)：9683-9697.
[11] Wei G, Li J, Wang N, et al. Spatial abundance and diversity of Bacterioplankton in a typical stream-forming ecosystem, Huangqian Reservoir, China[J]. J Microbiol Biotechnol, 2014, 24(10)：1308-1318.
[12]Kirchman DL, Cottrel MT, DiTullio GR. Shaping of bacterial community composition and diversity by phytoplankton and salinity in the Delaware Estuary, USA[J]. Aquatic Microbial Ecology, 2017, 78(2)：93-106.
[13]Campbell BJ, Kirchman DL. Bacterial diversity, community structure and potential growth rates along an estuarine salinity gradient[J]. The ISME journal, 2013, 7(1)：210.
[14]Wang K, Ye X, Chen H, et al. Bacterial biogeography in the coastal waters of northern Zhejiang, East China Sea is highly controlled by spatially structured environmental gradients[J]. Environ Microbiol, 2015, 17(10)：3898-3913.
[15]Kim J, Kim HS, Han S, et al. Hydrodynamic effects on bacterial biofilm development in a microfluidic environment[J]. Lab on a Chip, 2013, 13(10)：1846-1849.
[16]Dong G F, Xie SQ, Zhu XM, et al. Nutri-toxicological effects of cyanobacteria on fish[J]. Acta Ecologica Sinica, 2012, 32(19)：6233-6241.
[17]Wang Z, Yang J, Zhou J, et al. Composition and structure of bacterial communities in waste water of aquatic products processing factories[J]. Research Journal of Biotechnology, 2014, 9(2)：65-70.
[18]Swan BK, Chaffin MD, Martinez-Garcia M, et al. Genomic and metabolic diversity of Marine Group I Thaumarchaeota in the mesopelagic of two subtropical gyres[J]. PLoS One, 2014, 9(4)：e95380.
[19]Finneran KT, Johnsen CV, Lovley DR. Rhodoferax ferrireducens sp. nov. , a psychrotolerant, facultatively anaerobic bacterium that oxidizes acetate with the reduction of Fe(III)[J]. International Journal of Systematic and Evolutionary Microbiology, 2003, 53(3)：669-673.
[20]Madigan MT, Jung DO, Woese CR, et al. Rhodoferax antarcticus sp. nov. , a moderately psychrophilic purple nonsulfur bacterium isolated from an Antarctic microbial mat[J]. Archives of Microbiology, 2000, 173(4)：269-277.
[21]Louca S, Parfrey LW, Doebeli M. Decoupling function and taxonomy in the global ocean microbiome[J]. Science, 2016, 353(6305)：1272-1277.
[22]Ma X, Zhao C, Gao Y, et al. Divergent taxonomic and functional responses of microbial communities to field simulation of aeolian soil erosion and deposition[J]. Molecular Ecology, 2017, 26(16)：4186-4196.
[23]Louca S, Jacques S, Pires APF, et al. Functional structure of the bromeliad tank microbiome is strongly shaped by local geochemical conditions[J]. Environ Microbiol, 2017, 19(8)：3132-3151.